大鼠椭圆囊再生毛细胞前体细胞来源的初步研究
The Origination of Progenitor Cells of Utricular Regenerative Hair Cells in Rat
刘俊;孔维佳;赵荣祥;
1:浙江省中医院耳鼻咽喉科
2:华中科技大学同济医学院附属协和医院耳鼻咽喉科





1Holley MC.Hair cell re-growth[J].International Journal of Pediatric Otorhinolaryngology,2003,67:S1.
2Romand R,Chardin S.Effects of growth factors on the hair cells after ototoxic treat ment of neonatal mammalian cochleain vitro[J].Brain Research,1999,825:46.
3Malgrange B,Belachew S,Thiry M,et al.Proliferation genera-tion of mammalian auditory hair cells in culture[J].Mecha-nisms of Development,2002,112:79.
4Kalinec F,Kalinec G,Boukhvalova M,et al.Establishment and characterization ofconditionallyimmortalized orgen of corti cell lines[J].Cell Biology International,1999,23:175.
5Corwin JT,Finley JE,Saffer LD,et al.Isolation of pure living hair cell epithelia by use thermolysin[J].Poster,1995,17:345.
6武忠弼.主编.病理学[M].第四版.北京:人民卫生出版社,1999.15~327.
7鄂征,主编.组织培养和分子细胞学技术[M].北京:北京出版社,2001.10~88
8Zheng JL,Lewis AK,Gao WQ.Establishment of conditionallyim-mortalized rat utricular epithelial cell lines using a retrovirus-me-diated gene transfer technique[J].Hear Res,1998,117:13.
9Zheng JL,Helbig C,Gao WQ.Induction of cell proliferation by fibroblast andinsulin-like growthfactorsin pure rat inner ear epithelial cell cultures[J].J Neurosci,1997,17:216
10Warchol ME,Lambert PR,Goldstein BJ,et al.Regenerative proliferationininner ear sensory epithelia fromadult guinea pigs and humans[J].Science,1993,259:1619.
11Lawlor P,Marcotti W,Rivolta MN,et al.Differentiation of mammalian vestibular hair cells fromconditionallyimmortal,postnatal supporting cells[J].J Neurosci,1999,19:9445.
12Gratton MA,Schulte BA,Hazen-Martin DJ.Characteriza-tion and development of aninner ear typeⅠfibrocyte cell cul-ture[J].Hear Res,1996,99:71.
13Yeh TH,Herman P,Martin F,et al.Primary culture of Re-issner’s membrane fromthe gerbil:growth,morphologic fea-tures,and characterization[J].Laryngoscope,1993,103:645.
14Doetzlhofer A,White PM,Johnson JE,et al.In vitro growth and differentiation of mammalian sensory hair cell progeni-tors:a requirement for EGF and periotic mesenchyme[J].Dev Biol,2004,272:432.
15Chen P,Segil N.p27(Kip1)links cell proliferation to mor-phogenesis in the developing organ of Corti[J].Develop-ment,1999,126:1581.
16Ozeki M,Duan L,Hamajima Y,et al.Establishment and characterization of rat progenitor hair cell lines[J].Hear Res,2003,179:43.
17Zheng JL,Gao WQ.Analysis of rat vestibular hair cell deve-lopment and regeneration using calretinin as an early marker[J].J Neurosci,1997,17:8270.
18刘晖,朱宏亮,李胜利,等.成年鼠耳蜗听觉细胞自然培养诱导毛细胞样细胞的产生[J].中华耳鼻咽喉科杂志,2004,39:438.
19Zhao HB.Long-termnatural culture of cochlear sensory epi-thelia of guinea pigs[J].Neuroscience Letters,2001,315:73.
20Keithley EM,Erkman L,Bennett T,et al.Effects of a hair cell transcription factor,Brn3.1,gene deletion on homozygous and heterozygous mouse cochleas in adulthood and aging[J].Hear Res,1999,134:71.
21Xiang M,Gao WQ,Hasson T,et al.Requirement for Brn-3c in maturation and survival,but not in fate determination of inner ear hair cells[J].Development,1998,125:3935.
22Luo L,Bennett T,Jung HH,et al.Developmental expression of alpha9acetylcholine receptor mRNAin the rat cochlea and vestibular inner ear[J].J Comp Neurol,1998,393:320.
23Morley BJ,Simmons DD.Developmental mRNA expression of the a9nicotinic acetylcholine receptor subunit in the rat cochlea[J].Developmental Brain Research,2002,139:87.
24Hasson T,Gillespie PG,Garcia JA,et al.Unconventional myosins ininner-ear sensory epithelia[J].J Cell Biol,1997,137:1287.
25Algrange B,Belachew S,Thiry M,et al.Proliferation genera-tion of mammalian auditory hair cells in culture[J].Mecha-nisms of Development,2002,112:79.
26Stone JS,Oesterle EC,Rubel EW.Recent insights into rege-neration of auditory and vestibular hair cells[J].Curr Opin Neurol,1998,11:17.
27李华伟,汪吉宝.内耳毛细胞的再生前体细胞的来源、激活及调节因素[J].听力学及言语疾病杂志,1998,6:51.
28Li L,Forge A.Morphological evidence for supporting cell to hair cell conversion in the mammalian utricular macula[J].Int J Dev Neurosci,1997,15:433.
29Tsue TT,Oesterle EC,Rubel EW.Hair cell regeneration in the inner ear[J].Otolaryngol Head Neck Surg,1994,111:281.