去神经支配后大鼠环杓后肌不同类型肌纤维的mRNA表达

The mRNA Expression of Different Muscle Fibre Types in Denervated Rat Musculus Cricoarytenoideus Posterior

聂明荣;张思毅;葛平江;刘少锋;陈少华;盛晓丽;

1:南方医科大学

2:广东省人民医院耳鼻喉科

摘要
目的研究去神经支配后大鼠环杓后肌不同类型肌纤维蛋白mRNA表达的变化。方法以切除单侧喉返神经后40天的8只大鼠的环杓后肌作为研究对象,使用实时定量PCR方法测定环杓后肌胶原III、肌纤维蛋白各亚型的mRNA表达,并与正常对照组相比较。结果去神经支配40天的大鼠环杓后肌,各亚型纤维蛋白的重链mRNA表达与正常对照组比较差异无统计学意义,但是I/IIB亚型纤维蛋白的mRNA表达的比值与对照组比较差异有统计学意义(P=0.018)。结论大鼠去神经支配40天时大鼠环杓后肌重链肌动蛋白I/IIB亚型纤维mRNA表达的比值增加,提示该肌肉的收缩力有减弱的趋势。
关键词
去神经;环杓后肌;逆转录聚合酶链反应
基金项目(Foundation):
广州市科技重大专项项目(2008a1-e4011-10)
作者
聂明荣;张思毅;葛平江;刘少锋;陈少华;盛晓丽;
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