前庭代偿过程中GABAA受体α1亚型在前庭内侧核中的变化

The Change of GABAA receptor α1 Subunit in the Medial Vestibular Nucleus following Unilateral Labyrinthectomy

邹宇;孔维佳;刘波;

1:广州医学院附属广州儿童医院耳鼻咽喉科

2:华中科技大学同济医学院附属协和医院耳鼻咽喉科

3:华中科技大学同济医学院附属协和医院耳鼻咽喉科 (广州510120)

摘要
目的观察单侧迷路破坏术后前庭内侧核(medial vestibular nuclei,MVN)内γ-氨基丁酸A受体(gamma-aminobutyric acid Areceptor,GABAA receptor)α1亚型的表达变化。方法将24只大鼠随机分为术后1、3、7天组和假手术组,每组6只,前三组切除大鼠一侧迷路,第4组行假手术,利用免疫组织化学、原位杂交组织化学的方法,观察术后1、3、7天组前庭内侧核区GABAA受体α1亚型表达的变化,及其在前庭代偿中可能的作用,并与假手术组进行比较。结果手术后1天、3天、7天组行为学上开始均有前庭静态症状,术后7天时前庭静态症状均消失,假手术组无相关症状出现。免疫组织化学、原位杂交组织化学方法前庭内侧核中各组手术侧与对侧比较及术后1天、3天、7天组与假手术组比较,GABAA受体α1亚型表达差异均无统计学意义(P>0.05)。结论在前庭代偿的早期,GABAA受体α1亚型表达的变化可能没有涉及前庭代偿。
关键词
前庭内侧核;前庭代偿;γ-氨基丁酸A受体
基金项目(Foundation):
国家杰出青年基金(编号39925035);; 卫生部临床学科重点项目基金(编号2004468)资助
作者
邹宇;孔维佳;刘波;
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