双侧耳蜗毁损后大鼠听皮层突触素表达的变化

Expression of Synaptophysin in the Auditory Cortex in Rats with Bilateral Cochlear Ablation

李孟;华清泉;廖华;肖伯奎;

1:武汉大学人民医院耳鼻咽喉-头颈外科

摘要
目的观察双侧耳蜗毁损后大鼠听皮层(auditory cortex,AC)突触素表达水平的变化。方法30只SD大鼠随机均分为6组:双侧耳蜗毁损后3、7、14、21、30天组及假手术对照组,每组各5只。双侧耳蜗毁损后不同时间行听皮层突触素二步法免疫组化染色,观察突触素表达的变化。结果双侧耳蜗毁损后3天突触素表达增高,毁损后7天突触素表达较毁损后3天有所下降,毁损后14、21天突触素表达持续上升,21天时上升到最高峰,毁损后30天突触素表达开始下降,除毁损后7天组外,其余各组与假手术对照组差异均有统计学意义。各组左右耳表达差异均无统计学意义。结论大鼠双侧耳蜗去传入损伤后,突触素表达的动态变化反映了听皮层内神经元突触的重塑情况。
关键词
听觉可塑性;耳蜗毁损;听皮层;突触素
基金项目(Foundation):
国家自然基金面上项目资助(编号30772397)
作者
李孟;华清泉;廖华;肖伯奎;
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