耳蜗缺血损伤动物模型的建立

A Guinea Pig Model of Cochlear Ischemia

冯彪;邱建华;

1:西安第四军医大学西京医院全军耳鼻咽喉头颈外科中心

摘要

目的探讨建立一种耳蜗缺血动物模型的方法。方法将30只豚鼠随机分为3组:正常组、手术组、血栓组,每组10只。血栓组通过手术经颅底暴露小脑前下动脉(anterior inferior cerebellar artery,AICA),使用40%三氯化铁(FeCl3)溶液诱导AICA形成血栓;手术组仅完成暴露豚鼠小脑前下动脉;正常组未作特殊处理。手术完成后饲养动物1周,实验过程中采用激光多普勒血流量仪监测耳蜗底回血流量(cochleablood flow,CoBF),实验前后及术后1周测试三组豚鼠听性脑干反应(ABR)波Ⅲ反应阈,血栓组将AICA组织切片HE染色观察血栓形态。结果手术组和血栓组动物均存活730天,血栓组在FeCl3诱导后,CoBF值下降至诱导前约50%,ABR波Ⅲ反应阈均比实验前有明显提高,正常组和手术组实验前后差异无统计学意义。AICA切片HE染色,可见血栓组AICA有明显白色血栓形成,成分为纤维素、血小板及少量红细胞。结论应用FeCl3能够成功诱导小脑前下动脉血栓形成,可制备耳蜗缺血损伤模型;动物能够存活1周以上,最长达30天,是一种慢性耳蜗缺血损伤模型研究的新尝试,为临床研究慢性内耳微循环障碍疾病提供较理想的实验动物模型。

关键词

动物模型;慢性缺血;耳蜗;三氯化铁;动脉血栓;耳蜗血流量

基金项目(Foundation):

国家自然科学基金资助项目(项目编号:39200133)

作者

冯彪;邱建华;

参考文献

1B hme G.Hearing disordersin peripheral arterial vascular dis-eases.Acontribution on hearinglossinthe aged[J].Laryngol Rhinol Otol(Stuttg),1987,66:638.

2Lee H,Sohn SI,Jung DK.Sudden deafness and anterior infe-rior cerebellar artery infarction[J].Stroke,2002,33:2807.

3Quirk WS,Seidman MD.Cochlear vascular changes in re-sponse to loud noise[J].AmJ Otol,1995,16:322.

4Chao TK.Sudden sensorineural hearingloss after rapid reduc-tion of blood pressurein malignant hypertension[J].Ann Otol Rhinol Laryngol,2004,113:73.

5Nuti D,Cosentino G,Vessio G.Total cholesterol and high-density lipoprotein cholesterol in sensorineural deafness[J].Quad Sclavo Diagn,1983,19:129.

6熊巍,邱建华,乔莉,等.血栓溶栓方法制备耳蜗缺血再灌注动物模型[J].中华耳科学杂志,2005,3:186.

7罗志强,张先锋,彭华,等.三氯化铁诱导建立基底动脉栓塞动物模型[J].临床耳鼻咽喉科杂志,2003,17:364.

8Tsuji S,Tabuchi K,Hara A,et al.Long-termobservations on the reversibility of cochlear dysfunction after transient is-chemia[J].Hear Res,2002,166:72.

9Hu BH,Chang JS,Rui G.Effects of carbogen on decreasesin endocochlear potential and cochlear microcirculationinduced by ischemia of the cochlea[J].Acta Otolaryngol JT-Acta oto-laryngologica,1993,113:720.

10胡博华,姜泗长,顾瑞.耳蜗缺血的动物实验模型[J].微循环学杂志,1994,4:26.

11李学佩,马铁民,马芙蓉,等.椎基底动脉脑缺血动物模型[J].北京医科大学学报,1995,6:427.

12Seidman MD,Quirk WS,Nuttall AL,et al.The protective effects of allopurinol and superoxide dismutase-polyethylene glycol onischemic and reperfusion-induced cochlear damage[J].Otolaryngol Head Neck Surg,1991,105:457.

13Levine RA,Bu-Saba N,Brown MC.Laser-Doppler mea-surements and electrocochleography during ischemia of the guinea pig cochlea:implications for hearing preservationin a-coustic neuroma surgery[J].Ann Otol Rhinol Laryngol,1993,102:127.

14Giebel W,Str mer H,Arold R.Experimental studies of is-chemia of the cochlea[J].Laryngorhinootologie,1992,71:161.

15张学渊,汪吉宝.光化学建立豚鼠耳蜗微循环障碍模型的初步报告[J].中华耳鼻咽喉科杂志,1995,30:285.

16Kurz KD,Main BW,Sandusky GE.Rat model of arterial thrombosis induced by ferric chloride[J].Thromb Res,1990,60:269.

17Konstantinides S,Schafer K,Thinnes T,et al.Plasminogen activator inhibitor-1andits cofactor vitronectin stabilize ar-terial thrombi after vascular injury in mice[J].Circulation,2001,103:576.

本文信息

PDF(139K)

本文关键词相关文章

动物模型慢性缺血耳蜗三氯化铁动脉血栓耳蜗血流量

本文作者相关文章

冯彪邱建华