大鼠耳蜗毛细胞前体细胞与耳蜗间质细胞共培养的实验研究

Co-culture of Hair Cell Progenitors and Mesenchymal Cells from Rat Cochlea

张媛;郭维;胡吟燕;郑贵亮;翟所强;

1:中国人民解放军总医院耳鼻咽喉科研究所

2:中国人民解放军总医院耳鼻咽喉科研究所

3:中国人民解放军总医院耳鼻咽喉科研究所

4:中国人民解放军总医院耳鼻咽喉科研究所

摘要
目的探讨耳蜗间质细胞对耳蜗毛细胞前体细胞——大上皮嵴(greater epithelial ridge,GER)细胞的诱导分化作用。方法取出生后第1天大鼠20只,随机分为2组,每组各10只,利用酶消化和机械分离相结合的方法分别分离出纯耳蜗GER细胞和耳蜗间质细胞,并进行体外混合培养及形态学的观测,对共培养10天的标本行抗Calretinin(标记幼稚毛细胞)及抗p27抗体(标记支持细胞)的免疫细胞组织化学染色鉴定。结果混合培养的耳蜗GER细胞和间质细胞易形成片状细胞岛,共培养10天后,有部分细胞表达Calretinin,亦有少数细胞表达p27,但无Calretinin及p27双标记者。结论GER细胞与耳蜗间质细胞共培养,可以诱导GER细胞分化为毛细胞及支持细胞表型。
关键词
毛细胞再生;前体细胞;大上皮嵴;间质细胞
基金项目(Foundation):
国家自然科学基金项目(编号30440063);; 海外青年学者合作研究基金项目(编号30228029);; 北京市自然科学基金项目(编号7053076)
作者
张媛;郭维;胡吟燕;郑贵亮;翟所强;
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