应用LsAB技术显示五羟色胺受体亚型1A在小鼠螺旋神经元上的分布

Application of LsAB Technique to identifying Serotonin Receptor 1A in the Spiral Ganglion

李刚;唐玥玓;程薇波;

1:四川大学华西医院耳鼻咽喉科

2:四川大学华西医院耳鼻咽喉科

3:四川大学公共卫生学院病理学教研室 成都610041

4:成都610041

摘要
目的显示五羟色胺(serotonin,5-HT)受体亚型1A在螺旋神经元上的分布。方法采用出生后3~6天的乳小鼠耳蜗进行冰冻切片,应用酶标链亲和素-生物素(labeled streptavidin-biolin technique,LsAB)技术显示五羟色胺受体亚型1A在螺旋神经元上的分布。结果实验组的乳鼠耳蜗螺旋神经元的细胞膜显示阳性信号,阴性对照组的乳鼠耳蜗螺旋神经元的细胞膜上未发现阳性信号。结论五羟色胺受体亚型1A存在于耳蜗螺旋神经元的细胞膜上,推测当毛细胞释放兴奋性神经递质引起螺旋神经元兴奋时,五羟色胺作为神经递质对螺旋神经元的听觉传导活动起着重要的调节作用。
关键词
酶标链亲和素-生物素技术;五羟色胺受体亚型1A;耳蜗;螺旋神经元
基金项目(Foundation):
国家自然科学基金课题资助(编号30471880)
作者
李刚;唐玥玓;程薇波;
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