噪声对豚鼠耳蜗外淋巴Ca~(2+)的影响及天麻素对噪声性聋的防护作用

Alteration of the Calcium Content in Perilymph of the Cochlea of the Guinea Pig after Noise Exposure with and without Application of the Gastrodin

崔彩霞;赵荣祥;

1:杭州师范大学附属医院耳鼻咽喉科

2:浙江中医药大学附属一院耳鼻咽喉科 (杭州310015)

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摘要

目的探讨噪声暴露对耳蜗外淋巴Ca2+含量的影响,并观察天麻素对噪声性聋的防护作用。方法将42只健康豚鼠随机分为3组,8只为对照组,未作噪声暴露,17只为噪声暴露组,17只为噪声+天麻素组,用天麻素预防,用微量进样-火焰原子吸收光谱法测定耳蜗外淋巴Ca2+含量,检测噪声暴露前、后听性脑干反应(ABR),并与对照组比较。结果噪声暴露组耳蜗外淋巴平均Ca2+含量(113.67±42.22μg/ml)显著低于对照组(196.91±47.10μg/ml)(P<0.01),噪声+天麻素组耳蜗外淋巴平均Ca2+含量(151.15±60.65μg/ml)与对照组差异无统计学意义(P>0.05);噪声暴露后,噪声+天麻素组、噪声暴露组ABR阈值均显著升高(P<0.01),但噪声暴露组(88.53±5.08dBSPL)高于噪声+天麻素组(83.68±4.93dBSPL),差异有统计学意义(P<0.05)。结论噪声暴露可降低耳蜗外淋巴Ca2+含量,后者是噪声致聋的重要发病机理之一;天麻素能减轻噪声暴露引起的外淋巴Ca2+下降,对噪声性聋可能具有一定的预防作用。

关键词

天麻素;噪声性聋;外淋巴;Ca2+

基金项目(Foundation):

浙江省中医药管理局资助项目(编号2003C044A)

作者

崔彩霞;赵荣祥;

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崔彩霞赵荣祥