正常大鼠听觉中枢四种氨基酸类神经递质测定

Distribution of Four Amino Acid Neurotransmitters in Central Auditory System of Rats

肖伯奎;华清泉;王勤瑛;

1:武汉大学人民医院耳鼻咽喉-头颈外科

2:武汉大学人民医院耳鼻咽喉-头颈外科

3:武汉大学人民医院耳鼻咽喉-头颈外科 武汉430060

4:武汉430060

摘要
目的探讨并比较听觉中枢四种氨基酸类神经递质的含量。方法选取成年SD大鼠20只,采用日立835-50型氨基酸自动分析仪分别测定其耳蜗核、下丘和听皮层中r-氨基丁酸(gamma-aminobutyric acid,GABA)、甘氨酸(glycine,Gly)、谷氨酸(glutamic acid,Glu)及天冬氨酸(aspartate,Asp)的含量。结果听皮层与耳蜗核相比,GABA的含量差异无统计学意义(P>0.05),听皮层、耳蜗核分别与下丘相比,其含量差异均有显著统计学意义(P<0.01);听皮层与下丘相比,Gly的含量差异无统计学意义(P>0.05),听皮层、下丘分别与耳蜗核相比,其含量差异均有显著统计学意义(P<0.01);下丘与耳蜗核相比,Glu的含量差异无统计学意义(P>0.05),下丘、耳蜗核分别与听皮层相比,其差异均有显著统计学意义(P<0.01);听皮层、下丘及耳蜗核三者间相互比较,Asp的含量差异均无统计学意义(P>0.05)。结论听觉中枢的不同部位GABA、Gly及Glu的含量各异,可能与其神经元的活性不同有关,反映了脑神经元的功能状态。
关键词
脑;神经元;氨基酸
基金项目(Foundation):
作者
肖伯奎;华清泉;王勤瑛;
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肖伯奎华清泉王勤瑛