SOX5、SOX6基因在豚鼠耳蜗中的表达及意义

Analysis of RT-PCR on the Expression of SOX5,SOX6 Genes in Guinea Pig Cochlea

袁虎;韩东一;郭维;王秋菊;

1:解放军总医院耳鼻咽喉-头颈外科 解放军耳鼻咽喉科研究所

2:解放军总医院耳鼻咽喉-头颈外科

3:解放军耳鼻咽喉科研究所

4:解放军总医院耳鼻咽喉-头颈外科

摘要
目的探讨SOX5、SOX6基因在豚鼠耳蜗中的表达及该基因对耳蜗发育的影响与作用。方法活体解剖取出豚鼠耳蜗、肝脏、脑、肾脏和心脏等组织,应用Primer 5.0软件设计SOX5、SOX6引物。使用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)法检测SOX5、SOX6基因的表达情况。结果SOX5基因在不同组织中均有表达,耳蜗中的表达相对较弱。SOX5和SOX6基因均在耳蜗组织表达。结论SOX5、SOX6基因的表达具有一定的组织特异性,两者均在耳蜗中的表达提示SOX5、SOX6基因对耳蜗的骨性组织发育可能存在一定作用。
关键词
基因表达;逆转录-聚合酶链反应;SOX5;SOX6;耳蜗
基金项目(Foundation):
国家自然基金面上项目(编号30370782&30470956);; 全国优秀博士论文作者专项基金(No.200463)联合资助
作者
袁虎;韩东一;郭维;王秋菊;
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袁虎韩东一郭维王秋菊