新生小鼠膜迷路耳蜗外侧壁的组织培养

Tissue Culture of Lateral Cochlear Membrane Labyrinth in Neonatal Mouse

李洪涛;刘兆华;王锦玲;

1:第四军医大学西京医院耳鼻咽喉科

2:第三军医大学大坪医院复合伤研究所

3:第四军医大学西京医院耳鼻咽喉科 西安710032

4:西安710032

摘要
目的培养小鼠耳蜗螺旋韧带/血管纹来源的细胞,为体外研究提供细胞模型。方法显微解剖新生小鼠耳蜗螺旋韧带/血管纹组织,组织块外植培养,胰蛋白酶消化分离细胞,免疫组织化学染色,原位透射电镜观察鉴别细胞的来源。结果自外植耳蜗螺旋韧带/血管纹组织生长出上皮样细胞及成纤维样细胞。前者呈典型的上皮细胞形态,表达细胞角蛋白,并具血管纹边缘细胞的超微结构特征。后者呈成纤维细胞形态,表达波形蛋白,具有耳蜗螺旋韧带成纤维细胞的超微结构特征。结论培养出小鼠耳蜗血管纹边缘细胞及螺旋韧带成纤维细胞来源的原代上皮细胞及传代成纤维细胞,为体外研究提供了细胞模型及方法。
关键词
膜迷路;组织培养;血管纹边缘细胞;螺旋韧带成纤维细胞
基金项目(Foundation):
作者
李洪涛;刘兆华;王锦玲;
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