N型乙酰胆碱受体α10亚基在大鼠内耳耳蜗及前庭中的表达

Expression of α10 Acetylcholine Receptor Subunit mRNA in the Rat Cochlea and Vestibular

姚琦;孔维佳;程华茂;吴婷婷;夏交;朱云;

1:华中科技大学同济医学院附属协和医院耳鼻咽喉科

2:华中科技大学同济医学院附属协和医院耳鼻咽喉科

3:华中科技大学同济医学院附属协和医院耳鼻咽喉科

4:华中科技大学同济医学院附属协和医院耳鼻咽喉科

摘要
目的研究N型乙酰胆碱受体α10亚基在大鼠前庭终器、Scarpa’s神经节、基底膜、螺旋神经节中的表达。方法运用RT-PCR技术分别检测大鼠前庭终器、Scarpa’s神经节、基底膜、螺旋神经节编码N型乙酰胆碱受体α10亚基的基因表达。以大鼠脑组织RNA为阳性对照。结果N型乙酰胆碱受体α10亚基在大鼠前庭终器、基底膜上有表达,在Scarpa’s神经节、螺旋神经节中无表达。结论在前庭终器和基底膜上扩增出标志N型乙酰胆碱受体α10亚基基因表达的PCR产物,为乙酰胆碱是内耳重要的神经递质之一提供了支持,并为N型乙酰胆碱受体在内耳中的传入-传出调节中的作用提供了依据。
关键词
N型乙酰胆碱受体α10;耳蜗;前庭;逆转录-聚合酶链反应技术;大鼠
基金项目(Foundation):
国家杰出青年科学基金(39925035);; 卫生部临床学科重点项目资助(20012332);; 国家自然科学基金(30070809);; 高等学校博士学科点专项科研基金(20020487062)资助项目
作者
姚琦;孔维佳;程华茂;吴婷婷;夏交;朱云;
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