尼莫地平对内耳缺血/再灌注豚鼠耳蜗血迷路屏障通透性的影响

Effect of Nimodipine on Blood-Labyrinth Barrier Permeability Following Cochlear Ischemia/Reperfusion Injury in Guinea Pigs

权博源;邱建华;刘顺利;

1:第四军医大学西京医院全军耳鼻咽喉专科中心

2:第四军医大学西京医院全军耳鼻咽喉专科中心

3:第四军医大学西京医院全军耳鼻咽喉专科中心西安710032

4:西安710032

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摘要

目的研究尼莫地平对内耳缺血/再灌注后豚鼠耳蜗血迷路屏障通透性的影响。方法随机将32只豚鼠分为4组,每组8只,分别为缺血用药组、缺血对照组、再灌注用药组、再灌注对照组。采用小脑前下动脉FeCl3诱导血栓形成制备耳蜗缺血豚鼠模型,缺血1小时后再经股静脉给予尿激酶溶解血栓制备再灌注模型,分别于缺血后早期及再灌注后静脉注射尼莫地平,各对照组以等量生理盐水代替尼莫地平注射。采用激光多普勒血流测量仪监测耳蜗血流量(cochlear bloodflow,CoBF),测量实验前后豚鼠听性脑干反应(ABR)波Ⅲ反应阈值;分别在股静脉推注伊文思蓝2小时后采用荧光定量检测其血迷路屏障通透性变化。结果豚鼠耳蜗发生缺血后早期应用尼莫地平组与缺血对照组伊文思蓝通过血迷路屏障的平均量分别为每只0.245±0.108和0.442±0.153μg,差异有统计学意义(P<0.05);耳蜗发生缺血再灌注后应用尼莫地平组与再灌注对照组,伊文思蓝通过血迷路屏障的平均量分别为每只0.963±0.261和0.620±0.148μg,差异有统计学意义(P<0.05)。结论在内耳缺血早期使用尼莫地平,能减轻血迷路屏障的损伤;但在缺血再灌注后使用尼莫地平,则加重血迷路屏障的损伤,使其通透性增加。

关键词

尼莫地平;缺血/再灌注;内耳;毛细血管通透性

基金项目(Foundation):

作者

权博源;邱建华;刘顺利;

参考文献

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权博源邱建华刘顺利