小鼠耳蜗侧壁α_2 Na,K-ATPase在听觉及年龄相关性听力下降中的作用

The Auditory Functions of α_2 Na,K-ATPase in the Lateral Wall of Mice Cochlea and Its Relation with Age-related Hearing Loss

褚汉启;黄孝文;熊浩;韩芳;吴振恭;周良强;崔永华;

1:华中科技大学同济医学院附属同济医院耳鼻咽喉科

2:华中科技大学同济医学院附属同济医院耳鼻咽喉科

3:华中科技大学同济医学院附属同济医院耳鼻咽喉科

4:华中科技大学同济医学院附属同济医院耳鼻咽喉科

摘要
目的探讨耳蜗侧壁α2Na,K-ATPase通道在听觉功能和年龄相关性听力减退中的作用。方法利用半拷贝基因的α2Na,K-ATPase+/-杂合子小鼠作为试验平台,采用听性脑干反应(ABR)和耳蜗内电位(endoco-chlear potential,EP)检测方法,对α2Na,K-ATPase+/-杂合子小鼠和α2Na,K-ATPase+/+野生型小鼠进行ABR和EP检测,并对各个鼠龄段的小鼠进行ABR检测。结果α2Na,K-ATPase+/-杂合子小鼠的听力和EP值均低于α2Na,K-ATPase+/+野生型鼠;α2Na,K-ATPase+/-杂合子小鼠的听力随鼠龄增长而逐渐下降,高龄期小鼠的ABR阈值与其低龄时相比显著性升高(P<0.05)。结论耳蜗侧壁的α2Na,K-ATPase通道对听觉功能具有重要作用;携带半拷贝基因的αNa,K-ATPase+/-杂合子小鼠表现出轻度年龄相关性听力减退。
关键词
Na;K-ATPase;基因;耳蜗;离子通道;老年性聋
基金项目(Foundation):
国家自然科学基金资助项目(30371526);; 湖北省自然科学基金资助项目(2002AB127);; 教育部留学回国人员启动基金资助项目(教外司留2004-527号)
作者
褚汉启;黄孝文;熊浩;韩芳;吴振恭;周良强;崔永华;
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