前庭代偿过程中I组代谢性谷氨酸受体亚型在前庭内侧核中的变化

The Change of mGluR1 in the Medial Vestibular Nucleus following Unilateral Labyrinthectomy

张亚民;孔维佳;刘俊;胡钰娟;

1:华中科技大学同济医学院附属协和医院耳鼻咽喉科

2:华中科技大学同济医学院附属协和医院耳鼻咽喉科

3:华中科技大学同济医学院附属协和医院耳鼻咽喉科

4:华中科技大学同济医学院附属协和医院耳鼻咽喉科 武汉430022

摘要
目的观察单侧迷路破坏术后前庭内侧核(medialvestibular nuclei,MVN)内I组代谢性谷氨酸受体亚型(group I metabotropic glutamate receptors,mGluR1)的表达变化。方法成年雄性Wistar大鼠28只,分为迷路破坏组(20只)和对照组(8只),前者破坏单侧迷路,对照组手术方式相同但保持迷路完好。单侧迷路破坏术后,通过免疫组织化学、原位杂交组织化学法检测不同存活时间(术后12h、36h、7d、30d)两组动物MVN内mGluR1的表达变化。结果单侧迷路破坏术后可诱导同侧MVN区I组代谢性谷氨酸受体亚型mGluR1减少,术后12h最少,术后36h开始增加,至术后7d和30d后和对照组差异无统计学意义;对侧和术侧的变化趋势相同。结论单侧迷路破坏术后可诱导MVN区I组代谢性谷氨酸受体亚型mGluR1减少。初级前庭传入或中枢前庭神经元的静息放电降低可能与I组代谢性谷氨酸受体亚型mGluR1减少有关,但其在前庭代偿中的作用尚有待研究。
关键词
前庭代偿;前庭神经内侧核;大鼠;代谢性谷氨酸受体
基金项目(Foundation):
国家杰出青年基金(编号39925035);; 卫生部临床学科重点项目基金(编号2001321)资助
作者
张亚民;孔维佳;刘俊;胡钰娟;
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