豚鼠一侧迷路毁损后前庭内侧核c-fos表达

c-fos Expression in Medial Vestibular Nucleus of Guinea Pigs After Unilateral Labyrinthectomy

顾美珍;殷善开

1:上海交通大学附属第六人民医院耳科研究所

2:上海交通大学附属第六人民医院耳科研究所 上海200233

3:上海200233

摘要
目的 观察右侧迷路毁损后前庭内侧核 (medialvestibularnucleus ,MVN)早期快反应基因c -fos的表达特征。方法 选择健康白色赤目纯种豚鼠 2 7只 ,将豚鼠右侧迷路毁损后 ,在不同存活时间行前庭内侧核c -fos蛋白的二步法免疫组化染色 ,光学显微镜下观察染色结果。结果 对照组未见Fos样免疫反应。一侧迷路毁损 (u nilaterallabyrinthectomy ,UL)后可诱发双侧MVNFos样免疫反应 ,且术侧大于健侧。结论 ①UL毁损可诱导MVNFos样免疫反应增强 ,是前庭神经系统对外界刺激应答的一种方式 ,早期Fos样免疫反应 (1~ 6h)可能反应了复杂的神经通路的活性 ,延迟Fos样免疫反应 (2 4~ 4 8h)可能构成了前庭损伤后恢复过程中可塑性改变的基础 ;②前庭功能具有巨大的代偿潜力 ;③fos靶基因的本质及其他早期癌基因 (immediateearlygene,IEG)的作用仍待进一步研究。
关键词
前庭内侧核;c-fos;基因表达;单侧迷路毁损术
基金项目(Foundation):
作者
顾美珍;殷善开
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