首页 > 过刊浏览 > 文章详情

外源性谷氨酸引发的内耳毛细胞损伤与支持细胞GLAST表达的相关性研究

Relationship Between the Damage to IHC Initiated by Extraneous Glu and the Reaction of the Supporting Cell GLAST

屈涓;邱建华;刘顺利;刘津平

1:第四军医大学西京医院全军耳鼻咽喉专科中心

2:第四军医大学西京医院全军耳鼻咽喉专科中心

3:第四军医大学西京医院全军耳鼻咽喉专科中心

4:第四军医大学西京医院全军耳鼻咽喉专科中心西安710033

在线阅读

摘要

目的观察外源性谷氨酸引发的内耳毛细胞损伤与支持细胞谷氨酸转运体(glutamate-aspartate trans-porter,GLAST)表达的相关性。方法将30只成年健康豚鼠随机分为3组,每组10只,经耳蜗钻孔,第一组动物左耳为对照组,给予人工外淋巴液(对照组);第二组动物左耳给予20mmol/L谷氨酸(Glu组);第三组动物为正常组,不作特殊处理。采用听性脑干反应(ABR)测试、耳蜗铺片、硝酸银染色及免疫组织化学技术,观察Glu处理前后的听力学、形态学改变,以及引发的支持细胞GLAST的表达变化。结果给药后,三组豚鼠1kHz ABR平均阈值分别为8.00±2.68、18.00±3.50、8.00±2.58dB nHL;8kHz分别为10.00±3.33、27.00±4.22、7.50±2.64dB nHL;Glu组与对照组有显著差异(P<0.05)。形态学改变:给予20mmol/L谷氨酸3d后,外毛细胞有较明显改变,出现错乱、缺失,内毛细胞出现形态改变,与听力学变化一致。免疫组织化学:正常组GLAST在支持细胞有表达,给予Glu后GLAST强阳性表达。结论谷氨酸对豚鼠内耳毛细胞具有毒性作用,GLAST参与外源性谷氨酸的转运,并受细胞外谷氨酸浓度的调控。

关键词

谷氨酸转运体;毛细胞;突触;免疫组织化学

基金项目(Foundation):

作者

屈涓;邱建华;刘顺利;刘津平

参考文献

1Furness DN,Lehre KP.Immunocytochemical localization of the high affinity glutamate/aspartate transporter GLASTinthe rat and guinea-pig cochlea[J].Eur J Neurosci,1997,9:1961.

2Furness DN,Hulme JA,Lawton DM,et al.Distribution of the gluta-mate/aspartate transporter GLASTinrelationtothe afferent synapses of outer hair cells inthe guinea-pig cochlea[J].Joumal of Association for Researchin Otolaryngology,2002,3:234.

3Chaudhry FA,Lehre KP,Vanlookeren CM.Glutamate transporters in glial plasma membranes:highly differentiatedlocalizations revealed by quentita-tive ultrastructural immunocytochemistry[J].Neuron,1995,15:711.

4Lehre KP,Lery LM,Ottersen OP.Differential expression of two glial glutamate transporters in the rat brain:quantitative and immunocy-chemical observations[J].J Exp Biol,1994,196:237.

5Kanner BI.Sodium-coupled neurotransmitter transport:structure,function and regulation J[J].Exp Biol,1994,196:237.

6于宁,刘军,谭祖林,等.外源性谷氨酸对豚鼠耳蜗复合动作电位及微音电位的影响[J].解放军医学杂志,2002,27:239.

7张术,黄维国,邱建华,等.慢性次声暴露对豚鼠听阈及耳蜗毛细胞的影响[J].第四军医大学学报,2000,21:9.

8张术,黄维国,邱建华,等.慢性次声暴露对豚鼠耳蜗毛细胞听毛形态学的影响[J].第四军医大学学报,2000,23:1742.

9Ruel J,Chen C,Pujol R,et al.AMPA-preferring glutamate recep-tors in cochlear physiology of adult guinea-pig[J].J Physiol,1999,518:667.

10Glowatzki E,Fuchs PA.Transmitter release at the hair cell ribbon synapse[J].Nat Neurosci,2002,5:147.

11Onney JW.Excitotoxic amino acids[J].News Physiol Science,1986,1:19.

12Choi DW.Glutamate receptor andthe inducition of excitotoxic neuro-nal death[J].Prog Brain Res,1994,100:47.

13晏义平,孙凤艳.谷氨酸转运体的结构和功能[J].生命科学,1999,11:20.

14Eybalin M,Pujol R.Aradioautographic study of[3H]L-glutamate and[3H]L-glutamine uptake in the guinea-pig cochlea[J].Neu-roscience,1983,9:863.

15Usami S,Ottersen OP.The localization of taurine-like immunoreac-tivityinthe organ of Corti:a semiquantitative,post-embeddingim-muno-electron microscopic analysisinthe rat withsome observations inthe guinea pig[J].Brain Res,1995,676:285.

16Usami S,Osen KK,Zhang N,et al.Distribution of glutamate-like and glutamine-like immunoreactivities in the rat organ of Corti:a light microscopic and semiquantitative electron microscopic analysis with a note onthe localization of aspartate[J].Exp Brain Res,1992;91:1.

17Seal RP,Amara SG.Excitatory amino acid transporters:a family in flux[J].Annu Rev Pharmacol Toxicol,1999,39:431.

18Sims KD,Robinson MB.Expression patterns and regulation of glu-matate transporters in the developing and adult nervous system[J].Cri,Rev,Neurobiol,1999,13:169.

19Tanaka K.Cloning and expression of a glutamate transporter from mouse brain[J].Neurosci Lett,1993,159:183.

20Danbolt NC.Glutamate uptake.Porg[J].Neurobiol,2001,65:1.

21Kanai Y,Smith CP,Hediger MA.Anewfamily of neurotransmitter transporters:the high-affinity glutamate transporters[J].The FASEBJ,1993,7:1450.

本文信息

PDF(551k)

本文关键词相关文章

谷氨酸转运体毛细胞突触免疫组织化学

本文作者相关文章

屈涓邱建华刘顺利刘津平