基质金属蛋白酶-2及其抑制剂和白细胞介素-6在中耳胆脂瘤的表达及意义

Expression of IL-6 and Matrix Metalloproteinase-2 in Human Middle Ear Cholesteatoma

汪欣;祝威;王苹;郭晓峰;张影

1:吉林大学第一医院耳鼻咽喉-头颈外科

2:吉林大学第一医院耳鼻咽喉-头颈外科

3:吉林大学第一医院耳鼻咽喉-头颈外科

4:吉林大学第一医院耳鼻咽喉-头颈外科

摘要
目的研究基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及其抑制剂(tissue inhibitor of met-alloproteinase,TIMP-2)和白细胞介素-6(interleukin-6,IL-6)在中耳胆脂瘤中的表达,探讨其表达在胆脂瘤溶骨机制中的作用。方法应用免疫组化技术检测32例患者中耳胆脂瘤组织和10例正常人外耳道皮肤组织中MMP-2、TIMP-2和IL-6的表达。结果MMP-2在胆脂瘤上皮各层细胞中均有表达,基底层表达最强,棘层次之,颗粒层及角质层细胞表达较弱;TIMP-2在基底细胞层和棘细胞层有少量表达;IL-6的表达分布于胆脂瘤上皮全层,但在基底层细胞表达最弱,棘细胞层、颗粒层和角质层依次递增,染色最强的部位是颗粒层和角质层,而正常外耳道皮肤未见MMP-2、TIMP-2、IL-6的表达。结论在胆脂瘤组织中存在MMP-2与TIMP-2的表达失衡,导致胆脂瘤的骨质破坏。同时胆脂瘤上皮高表达IL-6可能是MMPs过表达的原因。
关键词
中耳胆脂瘤;基质金属蛋白酶;基质金属蛋白酶抑制剂;白细胞介素-6
基金项目(Foundation):
作者
汪欣;祝威;王苹;郭晓峰;张影
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