耳蜗血管交感及感觉神经双重调节的形态学证据

Morphological Evidences of Sympathetic Nerve and Sensory Nerve Regulating Cochlear Vessels

邱建华;乔莉;刘顺利

1:第四军医大学西京医院耳鼻咽喉科全军耳鼻咽喉专科中心

2:第四军医大学西京医院耳鼻咽喉科全军耳鼻咽喉专科中心

3:第四军医大学西京医院耳鼻咽喉科全军耳鼻咽喉专科中心 西安710032

4:西安710032

摘要
目的 研究酪氨酸羟化酶 (tyrosinehydroxylase,TH)和钙基因相关肽 (calcitoningene -relatedpeptide ,CGRP)在蜗轴螺旋动脉 (spiralmodiolarartery ,SMA)的定位及共存。方法 解剖显微镜下分离豚鼠SMA及其分支。采用TH及CGRP抗体及免疫组织化学ABC法和双标免疫荧光共聚焦定位技术。结果 TH、CGRP阳性的神经纤维分布于SMA的全长及其分支 ,两者均呈神经纤维网包绕SMA。标记的神经纤维有明显的分支和交叉 ,含明显的膨体 ,直径差异较大。纤维的密度随动脉走行逐渐降低。荧光双标共聚焦显微镜观察显示 ,在豚鼠的SMA ,TH、CGRP免疫反应阳性的神经纤维部分共存。结论 CGRP和肾上腺素能神经纤维共同参予对耳蜗血流的调节。
关键词
蜗轴螺旋动脉;酪氨酸羟化酶;钙基因相关肽
基金项目(Foundation):
作者
邱建华;乔莉;刘顺利
参考文献

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