外周听觉活性改变诱导在体听皮层突触NMDA受体表达变化

The Changes of Peripheric Auditory Activity Induced the Expression of Synaptic NMDA Receptors in Auditory cortex in Vivo

阮清伟;王正敏

1:复旦大学附属眼耳鼻喉科医院耳鼻咽喉科

2:复旦大学附属眼耳鼻喉科医院耳鼻咽喉科上海200031

3:上海200031

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摘要

目的　观察听觉剥夺和耳蜗电刺激对听皮质突触N -甲基 -D -天冬氨酸受体 (N -methyl-D -as partatereceptor,NMDAR)表达的影响。方法　经两次不连续梯度超速离心 ,从初级听皮质提取高度纯化的突触体后 ,采用Westernblotting对耳毒性聋组、听力正常组及电耳蜗内刺激 0 .5～ 2h的各实验组听皮质突触体NMDAR三个亚型的表达进行比较。结果　证实了听觉剥夺的成年或发育期大鼠耳蜗电刺激仅仅 1.5小时可以诱导突触体NR2A蛋白的显著增加 ,但NR1和NR 2B蛋白量的改变并不显著。结论　在体大鼠的听皮质存在活性依赖的突触的NR2A蛋白的表达

关键词

突触体;初级听皮层;耳蜗电刺激;N-甲基-D-天冬氨酸受体;蛋白质合成;突触的可塑性

基金项目(Foundation):

作者

阮清伟;王正敏

参考文献

1　ScannevinRH ,HuganirRL .Postsynapticorganizationandregulationofexcitatorysynapses[J].NatRevNeurosci,2000,1:133.　

2　HearingH ,ShengM .Dendriticspines:structure,dynamicsandregu lation[J].NatRevNeurosci,2001,2:2.　

3　RookMS ,LuM ,KosikKS .CaMIIalpha3’untranslatedregion-di rectedmRNAtranslationinlivingneurons:visualizationbyGFPlink age[J].JNeurosci,2000,20:6385.　

4　CrinoP ,KhodakhahK ,BeckerK ,etal.Presenceandphosophoryla tionfactorsindevelopingdendrites[J].ProcNatlAcadSciUSA ,1998,95:2313.　

5　KacharminaJE ,JobC ,CrinoP ,etal.Stimulationofglutaminerecep torproteinsynthesisandmembraneinsertionwithinisolatedneuronaldendrites[J].ProcNatlAcadSciUSA ,2000,97:11545.　

6　AakaluG ,SmithWB ,NguyenN ,etal.Dynamicvisualizationoflocalproteinsynthesisinhippocampalneurons[J].Neuron,2001,30:489.　

7　QuinlanEM ,PhilpotBD ,HuganirRL ,etal.Rapid,experience-de pendentexpressionofsynapticNMDAreceptorsninvisualcortexinvivo[J].NatureNeuroscience,1999,2:352.　

8　WellsDG ,DongX ,QuinlanEM ,etal.AroleforthecytoplasmicpolyadenylationelementinNMDAreceptor-regulatedmRNAtransla tioninneurons[J].JNeurosci,2001,21:9541.　

9　BagniC ,MannucciL ,DottiCG ,etal.Chemicalstimulationofsynap tosomesmodulatesa-Ca2+ calmodulin-dependentproteinkinaseIImRNAassociationtopolysomes[J].JNeurosci,2000,20(RC76):1.　

10　ScheetzAJ,NairnAC ,Constantine-PatonM .NMDAreceptor-me diatedcontrolofproteinsynthesisatdevelopingsynapses[J].NatureNeuroscience,2000,3:211.　

11　ViciniS ,WangJF ,LiJH ,etal.Functionalandpharmacologicaldif ferencesbetweenrecombinantN -methyl-D -aspartatereceptors[J].JNeurophysiol,1998,79:555.　

12　IIIingRB .Activity-dependentplasticityintheadultauditorybrain stem[J].AudiologyNeuro-Otology,2001,16:319.　

13　IIIingRB ,MichlerSA .ModulationofP -CREBandexpressionofc-fosincochlearnucleusandsuperiorolivefollowingelectricalintra cochlearstimulation[J].Neuroreport,2001,12:875.　

14　HollingsworthEB ,McnealET ,BurtonJL ,etal.Biochemicalcharac terizationofafilteredsynaptoneurosomepreparationfromguineapigcerebralcortex:cyclicadenosine3’:5’-monophosphate-generat ingsystems,receptors,andenzymes[J].JNeurosci,1985,5:2240.　

15　DingledineR ,BorgesK ,BowieD ,etal.Theglutamatereceptorionchannels[J].PharmacolRev,1999,51:7.　

16　LiB ,ChenNS ,LuoT ,etal.Differentialregulationofsynapticandextra-synapticNMDAreceptors[J].Natureneuroscience,2002,5:833.　

17　SalviRJ ,WangJ ,DingD .Auditoryplasticityandhyperactivityfol lowingcocheardamage[J].HearingResearch,2000,147:261.　

18　DietrichV ,NieschalkM ,StollW ,etal.Corticalreorganizationinpatientswithhighfrequencycochlearhearingloss[J].HearingRe search,2001,158:95.　

19　IrvineDRF ,RajanR ,BrownM .Injury-anduse-relatedplasticityinadultauditorycortex[J].AudiologyNeuro-Otology,2001,6:192.

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突触体初级听皮层耳蜗电刺激 N-甲基-D-天冬氨酸受体蛋白质合成突触的可塑性

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阮清伟王正敏