小鼠耳蜗神经营养因子-3基因的克隆及其基因工程细胞模型的建立
Clone Mouse Cochlear Neurotrophin-3 Gene and Construce the Model of its Genetic Engieering Cells
高下;王锦玲;杨安钢;许彦鸣;王枫;高辉
1:第四军医大学西京医院耳鼻咽喉科
2:第四军医大学西京医院耳鼻咽喉科
3:第四军医大学基础部生物化学与分子生物学教研室
4:第四军医大学基础部生物化学与分子生物学教研室





1 Fritzsch B, Silos-Santiago I, Bianchi LM, et al. The role of neurotrophic factors in regulating the development of inner ear inervation[J]. Trends Neurosci,1997,20:159.
2 Fritzsch B, Pirvola U, Ylikoski J. Making and breaking the innervation of the ear nurotrophinic support during ear development and itsclinical implications[J]. Cell Tissue Res, 1999,295:369.
3 金冬雁,黎孟枫,译 分子克隆实验指南[M].第二版.北京:科 学出版社,1999.345-355.
4 张杰,汪家政 神经营养素家族的临床应用前景[J].军事医学 科学院院刊,1998,22:65.
5 孙建军,刘.神经生长因子家族与外周听觉神经系统[J].中 华耳鼻咽喉科杂志,1997,10:311
6 Staceker H, Gabaizadeh R, Federoff H, et aI. Brain-derived neu- rotrophic factor gene therapy prevents spiral ganglion degeneration after hair cells[J]. Otolaryngol Head Neck Surg,1998,119:7.
7 Shoji F, Miller AL, Mitchell A, et al. Differential protective effects of neurotrophins in the attenuation of noise-induced hair cells loss[J]. Hear Res,2000,146(1-2):134.
8 Agerman K, Canon B, Duan M, et al. Neurotrophins, NMDA recep- tors, and nitric oxide in development and protection of the auditory sys- tem[J]. Ann NY AcadSci,1999,884:131.
9 Ruan RS, Leong SK, Mark I, etal. Effects of BDNF and NT-3on hair cell survival in guinea pig cochlea damaged by kanamycin treat- ment[J]. Neuroreport,1999,l0:2067.
10 Van De Water Th, Staecker H, Halterman MW, et al. Gene therapy in the inner ear. Mechanisms and clinieal implications[J]. Ann N Y Acad Sci, 1999 , 884 : 344
11 Conrnetta K, Morgan HA, Anderson WF. Safety issues related to retroviral-mediated gene transfer in humans[J]. Human Gene Ther, 1991,2:5.