内耳免疫反应中内耳血管细胞粘附分子-1和α_4-整合素的表达

Expressi of Vascular Cell Adhesion Molecule-1 and α_4-Integrin During Inner Ear Immune Response

张程;黄魏宁;宋海涛;高波;周金梅

1:卫生部北京医院耳鼻咽喉科

2:卫生部北京医院耳鼻咽喉科

3:卫生部北京医院耳鼻咽喉科

4:卫生部北京医院耳鼻咽喉科

摘要
目的 探讨血管细胞粘附分子-1(vascular cell adhesion molecule-1,VCAM-1)和α4-整合素(α4-In-tegrin)在内耳免疫反应中的作用。方法 采用钥孔 血蓝蛋白激发已两次全身致敏的大鼠内耳,诱发其内耳免疫反应,制造迷路炎模型,然后通过免疫组织化学技术,检测内耳VCAM-1和α4-Integrin分别在内耳中的表达。结果 内耳激发后24小时,螺旋轴静脉(spiral modiolar vein,SMVs)及其回流小静脉(collecting venules,CVs)可见有VCAM-1表达,48小时达最高峰,维持此水平1周,然后下降。α4-Integrin在致敏后24小时即可表达于浸润于SMVs和CVs及蜗神经周围的白细胞,48小时达高峰,然后迅速下降。结论 内耳免疫反应时VCAM-1、α4-Integrin在炎性细胞从循环系统进入内耳的过程中发挥着重要作用。
关键词
迷路炎;内耳免疫;血管细胞粘附分子-1;α_4-整合素
基金项目(Foundation):
卫生部科学研究基金资助项目(92-2-043)
作者
张程;黄魏宁;宋海涛;高波;周金梅
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