豚鼠耳蜗─氧化氮合酶的分布

Distribution of NOS in the Cochlea of Guinea Pig

潘世恺;角南贵司子;山根英雄;王正敏

1:复旦大学医学院附属眼耳鼻喉科医院耳鼻咽喉科

2:日本大阪市立大学医学部耳鼻咽喉科教研室

3:日本大阪市立大学医学部耳鼻咽喉科教研室

4:复旦大学医学院附属眼耳鼻喉科医院耳鼻咽喉科 上海 200031

摘要
目的 用组织化学法,通过观察NADPH-黄递酶的活性了解一氧化氮合酶在豚鼠耳蜗内分布。方法 经4%多聚甲醛心脏灌注固定后,取出耳蜗,经3%依地酸脱钙后,作厚10μm冰冻切片,用辅酶Ⅱ孵育液在37℃条件下孵育1小时。结果 发现在耳蜗内、外毛细胞底部与耳蜗神经末梢接头处及毛细血管球内皮细胞有明显NADPH-黄递酶活性,此外在内、外柱细胞、支持细胞、血管纹及螺旋神经节细胞也有NADPH-黄递酶活性反应。结论 NO在维持耳蜗正常神经传导及毛细血管张力中起着重要作用。
关键词
一氧化氮合酶;还原型辅酶Ⅱ-黄递酶;耳蜗;组织化学;豚鼠
基金项目(Foundation):
作者
潘世恺;角南贵司子;山根英雄;王正敏
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