豚鼠发育过程中前庭上皮IGF-1及IGF-1R的表达变化

Expression and Function of IGF-1 and IGF-1R in Vestibular Epithelium During the Development of Guinea Pig

胡金玮;王锦玲;黄维国;姜鸿彦;邱建华;乔莉

1:第四军医大学西京医院耳鼻咽喉科

2:第四军医大学西京医院耳鼻咽喉科

3:第四军医大学西京医院耳鼻咽喉科

4:第四军医大学西京医院耳鼻咽喉科

摘要
目的检测胰岛素样生长因子-l(insulin-like growth factor-1,IGF-l)及其受体(insulin-like growth factor-1 receptor,ICF-1R)在豚鼠发育过程中前庭上皮的表达和分布特点,并探讨其生物学特性及意义。方法 利用免疫组织化学的方法,以IGF-1和ICF-1R抗体为标记物,检测发育期豚鼠前庭器中的表达情况及分布特点,以增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)检测细胞增殖变化。结果IGF-1及ICF-1R可在豚鼠前庭上皮细胞中表达,随年龄增加而逐渐减少;PCNA表达于支持细胞,随年龄增加,阳性细胞数逐渐减少。结论 豚鼠正常发育过程中前庭上皮中有ICF-l及IGF-1R的表达,为内源性的ICF-l通过旁分泌和自分泌方式在局部发挥其功能,壶腹嵴感觉上皮IGF-1及IGF-1R表达与细胞增殖相同步,IGF-1在发育早期是强烈的有丝分裂原,促进细胞增殖、分化。
关键词
前庭感觉上皮;胰岛素样生长因子-1;胰岛素样生长因子-1受体;细胞增殖
基金项目(Foundation):
国家自然科学基金资助项目(项目编号 39870788)
作者
胡金玮;王锦玲;黄维国;姜鸿彦;邱建华;乔莉
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