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Cu/ZnSOD基因敲除小鼠——研究耳蜗氧化损伤的理想模型

Cu/Zn Superoxide Dismutase Gene Knockout Mice——A Perfect Model for Cochlear Oxidative Damage

张欣欣;韩东一;丁大连;戴朴;杨伟炎;姜泗长;Richard J.Salvi

1:解放军总医院耳鼻咽喉科

2:解放军总医院耳鼻咽喉科

3:美国纽约州Buffalo大学听力及聋病康复中心

4:解放军总医院耳鼻咽喉科

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摘要

目的　确定一个理想的耳蜗氧化损伤模型。方法　对 5只Cu/ZnSOD基因敲除纯合突变 (Cu/ZnSOD无活性 ,Sod1- / - )小鼠和 10只Cu/ZnSOD杂合突变 (5 0 %Cu/ZnSOD失活 ,Sod1+/ - )小鼠及 5只野生小鼠 (Cu/Zn SOD活性正常 ,Sod1+/ +)进行ABR检测及内、外毛细胞计数。应用PCR技术检测Cu/ZnSOD基因敲除小鼠耳蜗mtDNA缺失情况。结果　Cu/ZnSOD基因敲除小鼠耳蜗内外毛细胞大量缺失 (P <0 .0 0 1) ,ABR检测 ,Sod1- / -小鼠ABR阈值在 8,16 ,32kHz及Sod1+/ -小鼠ABR阈值在 16 ,32kHz明显增大 (P <0 .0 0 1)。Cu/ZnSOD基因敲除小鼠耳蜗mtDNA有三种缺失 ,分别为mtDNA 386 7bp、mtDNA 372 6bp及mtDNA 432 6bp缺失。结论　Cu/ZnSOD基因敲除小鼠耳蜗在细胞及分子水平上均表现出受到ROS损伤的改变 ,是一个理想的耳蜗氧化损伤模型。

关键词

Cu/ZnSOD基因敲除小鼠;活性氧;模型;耳蜗氧化损伤

基金项目(Foundation):

国家杰出青年科学基金 (批准号 :3972 5 0 2 6 );; 中国博士后科学基金 (批准号 :中博基 [2 0 0 0 ] 2 3号 )资助

作者

张欣欣;韩东一;丁大连;戴朴;杨伟炎;姜泗长;Richard J.Salvi

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Cu/ZnSOD基因敲除小鼠活性氧模型耳蜗氧化损伤

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张欣欣韩东一丁大连戴朴杨伟炎姜泗长 Richard J.Salvi