碱性成纤维细胞生长因子对前庭毛细胞的保护作用

Protective Effect of Basic Fibroblast Growth Factor on Vestibular Hair Cells Injuryed by Neomycin in Vitro

熊敏;姜泗长;杨伟炎;张素珍;翟所强

1:广州军区广州总医院耳鼻咽喉科!广州510010

2:解放军总医院耳鼻咽喉科研究所

3:解放军总医院耳鼻咽喉科研究所

4:解放军总医院耳鼻咽喉科研究所

摘要
目的 观察碱性成纤维细胞生长因子 (basicFibroblastGrowthFactor,bFGF)对新霉素所致离体椭圆囊毛细胞损害的保护作用。方法 采用离体的豚鼠椭圆囊培养法 ,对照组为普通前庭培养液培养 ,实验组按培养液中含和不含bFGF分组 ,用新霉素造成各实验组培养的椭圆囊毛细胞损伤 ,各组椭圆囊均行扫描电镜检查并行毛细胞计数。结果 含bFGF的实验组毛细胞的存活数目显著高于不含bFGF的实验组 (P <0 .0 5 ) ,对照组毛细胞无缺失或损伤。结论 新霉素对离体的椭圆囊毛细胞有较强的破坏作用 ;bFGF对新霉素所致离体椭圆囊毛细胞的损伤有保护作用
关键词
碱性成纤维细胞生长因子;椭圆囊毛细胞;新霉素
基金项目(Foundation):
作者
熊敏;姜泗长;杨伟炎;张素珍;翟所强
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