卡那霉素耳中毒后豚鼠耳蜗热休克蛋白的表达

Expression of Heat Shock Protein 70 in Guinea Pigs Cochlea with Kanamycin Ototoxicity

陶泽璋;肖伯奎;刘剑锋;曲秋懿

1:湖北医科大学附一医院耳鼻咽喉-头颈外科!武汉

2:430060

3:湖北医科大学附一医院耳鼻咽喉-头颈外科!武汉

4:430060

摘要
目的 观察卡那霉素对热休克蛋白 70 (HSP70 )在豚鼠耳蜗中表达的影响。方法 取听力正常豚鼠随机分为实验组和对照组各 5只 ,分别给予 2 5 0mg·kg-1·d-1卡那霉素和生理盐水肌注 ,10天后处死。左耳铺片 ,右耳石蜡包埋切片。用免疫组织化学方法检测各组石蜡切片HSP70表达情况 ,通过计算机图像分析系统分析HSP70表达强度。结果 对照组中Corti器、血管纹、螺旋韧带、内螺旋缘、螺旋神经节HSP70表达呈弱阳性 ;耳蜗铺片显示内 ,外毛细胞正常。实验组中Corti器、血管纹、螺旋韧带、内螺旋缘HSP70表达呈强阳性 ,而在螺旋神经节呈弱阳性 ;耳蜗铺片显示外毛细胞大部分缺损。结论 卡那霉素能够诱导HSP70在豚鼠耳蜗中表达
关键词
卡那霉素;耳毒性;热休克蛋白;免疫组织化学
基金项目(Foundation):
作者
陶泽璋;肖伯奎;刘剑锋;曲秋懿
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