电针对急性后循环缺血豚鼠蜗神经核能量代谢功能重塑的影响及机制研究
Effect and Mechanism of Electroacupuncture on Energy Metabolism Remodeling of Cochlear Nucleus in Guinea Pigs with Acute Posterior Circulation Ischemia
王立宇;刘淑云;肖茂春;杨丽;殷泽登;
1:西南医科大学附属医院耳鼻咽喉头颈外科
摘要[Abstract] Objective To explore the effects of acute posterior circulation ischemia(PCD)on energy metabolism and SIRT1-PINK1-mediated mitochondrial autophagy in cochlear nucleus of guinea pigs, and the effect and mechanism of electroacupuncture. Methods A total of 40 healthy variegated guinea pigs were randomly divided into four groups, including operation group (OG,n=10)， control group (CG,n=10) , electroacupuncture group (EOG, n=10) and sham operation group (SOG,n=10). The right common carotid artery was surgically dissected and the right subclavian artery was ligated in the guinea pigs of OG to build PCI model. In the EOG. electroacupuncturing at Baihui and Neiguan points was performed for 30 minutes every day, and lasted for 7 days aftery the artery operation as well as OG. Moreover, the carotid exposure was the same as OG, and was not dissected and ligated in SOG.Micro PET/CT was used to detect the level in glucose metabolism in the cochlear nucleus. The uptake value of 18FFDG in the left and right cochlear nucleus was detected by Micro PET/CT,and the average ratio of SUVmax left/ right was analyzed in the cochlear nucleus.ELISA was used to detect the ATP level.The real-time quantitative PCR was used to detect the expression of PINK1,SIRT1and LC3 mRNA. Results Compared with the CG, the average ratio of SUVmax left/ right increased in the OG,and the ATP level decreased in the OG.Compared with the OG, the av- erage ratio of SUVmax left/ right decreased in the EOG,and the ATP level increased in the EOG. The expression of SIRT1 mRNA was lower in the EOG than that in the OG. There was no significant difference in the expression of PINK1 and LC3 mRNA among the 4 groups. Conclusion Electroacupuncturing at Baihui and Neiguan points may promote in the remodeling process of energy metabolism of cochlear nucleus in guinea pigs with acute PCI by regulating the expression of SIRT1.
关键词基金项目(Foundation):作者参考文献1 Sparaco M,Ciolli L,Zini A.Posterior circulation ischemic stroke-a review part II:imaging and acute treatment[J].Neurol Sci,2019,40(10):2007-2015.
2 Shin B,Cowan DB,Emani SM,et al.Mitochondrial transplantation in myocardial ischemia and reperfusion injury[J].Adv Exp Med Biol,2017,982:595-619.
3 李明熹，屈艺，母得志.线粒体自噬对新生大鼠缺氧缺血脑损伤的影响[J].中国当代儿科杂志，2017,19(2):242-249.
4 Tang BL.Sirt1 and the mitochondria[J].Mol Cells,2016,39(2):87-95.
5 Liu S,Yang Y,Mao X,et al.Improving glucose metabolism in the auditory cortex delays the aging of auditory function of guinea pig[J].Mech Ageing Dev,2020,190:111292.
6 刘阳，孙建宁，王堃，等.缺血性眩晕大鼠模型的建立[J].中国比较医学杂志，2013,23(1):10-13.
7 李莹莹，白玉龙.微型正电子发射计算机断层显像在脑缺血模型中的应用研究进展[J].中国康复理论与实践，2017,23(7):770-774.
8 邓虞娇，朱华，杨志，等.中枢神经系统临床用PET显像剂的研究进展[J].同位素，2020,33(4):250-262.
9 Pinchefsky EF,Schneider J,Basu S,et al.Nutrition and management of glycemia in neonates with neonatal encephalopathy treated with hypothermia[J].Semin Fetal Neonatal Med,https://DOI.org/10.1016/j.siny.2021.101268.Epub ahead of print.
10 Meng X,Tan J,Li M,et al.Sirt1:Role under the condition of ischemia/hypoxia[J].Cell Mol Neurobiol,2017,37(1):17-28.
11 Ravindran S,Kurian GA.Addressing the alterations in cerebral ischemia-reperfusion injury on the brain mitochondrial activity:a possible link to cognitive decline[J].Biochem Biophys Res Commun,2019,518(1):100-106.
12 赵汝舟，余志斌，张琳.缺氧预处理激活AMPK对大鼠心肌细胞缺氧/复氧损伤的保护作用[J].解放军医学杂志，2019,44(2):120-126.
13 马晓辰，薛明明.沉默信息调节因子1在动脉粥样硬化中的保护作用[J].世界最新医学信息文摘，2018,18(16):100-101,104.
14 Wan D,Zhou Y,Wang K,et al.Resveratrol provides neuroprotection by inhibiting phosphodiesterases and regulating the cAMP/AMPK/SIRT1 pathway after stroke in rats[J].Brain Res Bull,2016,121:255-262.
15 Zhu T,Xie WJ,Wang L,et al.Notoginsenoside R1 activates the NAMPT-NAD+-SIRT1 cascade to promote postischemic angiogenesis by modulating Notch signaling[J].Biomed Pharmacother,2021,140:111693.
16 Guo JM,Shu H,Wang L,et al.SIRT1-dependent AMPK pathway in the protection of estrogen against ischemic brain injury[J].CNS Neurosci Ther,2017,23(4):360-369.
17 贺平，颜虹，蒋素容，等.针刺大椎、人中、百会穴对脑缺血再灌注损伤大鼠脑线粒体超微结构的影响[J].湖南中医药大学学报，2018,38(1):55-58.
18 Tian WQ,Peng YG,Cui SY,et al.Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury[J].Chin J Integr Med,2015,21(8):618-623.
19 Khoury N,Koronowski KB,Young JI,et al.The NAD+-dependent family of sirtuins in cerebral ischemia and preconditioning[J].Antioxid Redox Signal,2018,28(8):691-710.