【Abstract] Objective To investigate the prevalence oftinnitus in medical staff, and the relevant influencing factors, in order to provide a scientific basis for the prevention and treatment of tinnitus in medical staff. Methods A multi-stage stratified cluster random sampling method was used to conduct a tinnitus epidemiological questionnaire survey on a total of 3 419 medical staff in Bazhong City, Sichuan and Wanzhou City, Chongqing, and the correlation analysis of possible influencing factors was carried out. Results The prevalence of tinnitus among medical staff in this study was 33. 1% (1 132/3 419) , and the prevalence of annoying tinnitus was 3.0% (103/3 419). The univariate analysis suggested educational background (χ²= 19.224,P<0.001)， professional title (χ²=6.383,P=0. 094), history of radiation exposure (χ²=4. 270, P=0.039) , history of drinking (χ²=8. 963, P=0.003),weekly working hours (χ²= 30. 462,P<0.001)， sleep quality (χ²=160.098, P<0.001), daily sleep time (χ² =34. 950, P<0.001), daily sleep duration (χ²=63. 096 ,P<0.001) were related to the prevalence of tinnitus. Binary logistic regression analysis showed that poor sleep quality increased the risk of tinnitus (OR=2.342， 95%CI 1.916~2.857,P<0.001). Sleep duration <7 hours per day increased the risk of tinnitus (OR=1.314，95%CI 1.064~1.623,P=0.011). The results of univariate analysis suggested that job title (χ²=6. 370, P=0.095), sleep quality(χ²=3.581,P=0.058), length of sleep per day (χ²=6. 418, P=0.011) were related to the prevalence of annoying tinnitus. Binary logistic regression analysis showed that sleep duration less than 7 hours a day increased the risk of annoying tinnitus (OR=1.792，95%CI 1.010~2.865，P=0.046).Conclusion Medical staff is a high-incidence group of tinnitus, but the chance of turning into annoying tinnitus is low. Poor sleep quality and insufficient sleep time are important risk factors for the onset of tinnitus and transformation into annoying tinnitus.