糖尿病性听力减退大鼠听觉神经通路相关蛋白质组学初步研究
Studies of Proteomics of the Auditory Neuropathway in Rats with Diabetes Related Hearing Impairment
欧阳思;杨径;杨念念;陈义;王继华
1:湖南省人民医院(湖南师范大学附属第一医院)耳鼻咽喉头颈外科
2:湖南长沙天心著文门诊部耳鼻喉科
3:邵阳市中心医院耳鼻咽喉科
摘要[Abstract] Objective To investigate the differential protcomics of the auditory neuropathway in rats with di- abetes-related hearing impairment. Methods 30 healthy male Wistar rats were randomly divided into the control group and the experimental group, with 15 rats in each group. The experimental group was injected with streptozotocin 55 mg/kg once through the tail vein to create the diabetes rat model, and the control group was injected with citric acid buffer in the same amount. After three months, tympanitis was excluded. Tested by ABR, Two-dimen- sional gel electrophoresis (2-DE) and Mass spectrometry (MS) were used to detect the different proteins in the auditory cortex and the cochlear nuclei between the normal rats (10 rats) and the diabetic rats (10 rats). Immunohistochemistry was used to identify the different proteins. Results The differences of protein expression in the auditory cortex and the cochlear nuclei between the normal rats and the diabetes rats were found, by means of identification of protein spots from 2-DE gels. Peptide mass fingerprints (PMF) maps identified 33 proteins (7 in cochlear nucleas and 16 in auditory cortex)，including Prohibitin， Synapsin,Cofilin-1，Annexin A3，T-complex protein 1, dihydropyrimidinase-related protein 2, Pyridoxal phosphate phosphatase, et al. The immunohistochemical study demonstrated that Prohibitin and Synapsin expression in the auditory cortex of the diabetes rats were decreased. Conclusion The discrepancy of the protein expression in the auditory cortex and the cochlear nuclei between the normal rats and the diabetes rats were found, which may be related to diabetes-related hearing impairment.
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