噪声暴露致听神经到耳蜗核神经通路电生理特性的改变

The Changes of the Electrophysiological Characteristics in the Pathway from Auditory Nerve to Cochlear Nucleus after Noise-exposure in Mouse

吴付连;冀旭颖;冯爽;孙昌志

1:广州市妇女儿童医疗中心耳鼻喉科

2:南方医科大学基础医学院生理学教研室

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摘要

目的通过研究噪声暴露致小鼠听力损伤后，耳蜗背侧核的兴奋性梭形神经元和耳蜗腹侧核的抑制性D型星形神经元接受听觉神经输入的电生理特性改变，探索噪声致听觉系统外周损伤后引起的中枢神经通路的变化。方法 30只健康成年的转基因小鼠SOM-tdTomato采用随机数字表法随机分为对照组(15只，不给予任何噪声暴露)、噪声暴露组(15只，120 dB SPL白噪声每天暴露4 h,连续暴露7 d)。应用听性脑干反应(ABR)测试两组小鼠的听功能，应用膜片钳离体脑片技术分析比较两组小鼠听觉神经到耳蜗核的梭形神经元和D型星形神经元投射通路电生理特性的改变；应用免疫荧光染色法确定记录神经元的类型。结果与对照组比较，噪声暴露组小鼠短声和短纯音ABR反应阈值升高，波Ⅲ潜伏期延长(P<0.05)。膜片钳离体脑片记录结果表明：两类神经元都表现有来自听神经的兴奋性突触后反应，与对照组比较，噪声暴露组反应幅度降低、概率下降、延时延长，其中对D型星形神经元的支配效率降低更多。结论听觉神经可以直接支配耳蜗核的梭形神经元和D型星形神经元；噪声损伤听力后，这种支配效率和强度均明显降低。

关键词

噪声暴露;听力损失;微环路;耳蜗核

基金项目(Foundation):

作者

吴付连;冀旭颖;冯爽;孙昌志

参考文献

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