艾塞那肽减轻卡那霉素加呋塞米诱导的小鼠螺旋神经元损伤

Exendin-4 Alleviates Spiral Neuron Injury Induced by Kanamycin and Furosemide in Mice

邬红霞;欧阳曦;范肖霞;戴光炆;赵旭旭;陈弢;康厚墉

1:重庆医科大学附属第一医院耳鼻咽喉头颈外科

摘要
目的 探讨高血糖素样肽-1受体(glucagon like peptide-1 receptor, GLP-1R)激动剂艾塞那肽(exendin-4)对卡那霉素加呋塞米诱导的小鼠螺旋神经元(spiral ganglion neurons, SGNs)损伤的作用。方法 原代培养的小鼠SGNs进行免疫荧光染色、小鼠耳蜗切片免疫组化染色,验证GLP-1R在SGNs上的表达。将30只C57BL/6J小鼠分成3组,每组10只,卡那霉素加呋塞米组一次性腹腔注射硫酸卡那霉素1 g/kg及呋塞米0.4 g/kg;艾塞那肽组卡那霉素和呋塞米注射前3 d开始腹腔注射艾塞那肽400μg/kg,每天1次,直到卡那霉素和呋塞米给药后第7 d;对照组腹腔注射等量生理盐水。分组处理后,使用HE染色观察SGN损伤情况并计数骨螺旋板里SGNs数量;采用Western blot技术检测各组耳蜗组织GLP-1R及凋亡相关蛋白Bax、Bcl-2、Cleaved-caspase-3的表达。结果 免疫荧光及免疫组化染色结果显示,GLP-1R表达于SGNs。HE染色显示,与对照组比较,卡那霉素加呋塞米组SGNs数量下降,艾塞那肽组SGNs数量有所恢复(P<0.05);Western blot结果显示,卡那霉素加呋塞米组GLP-1R蛋白表达下降,促凋亡蛋白Cleaved-caspase-3、Bax蛋白水平升高,抗凋亡蛋白Bcl-2表达水平下降;艾塞那肽组GLP-1R蛋白表达较卡那霉素加呋塞米组升高,Cleaved-caspase-3、Bax表达降低,Bcl-2表达升高(P<0.05)。结论 GLP-1R激动剂艾塞那肽可能通过减轻细胞凋亡,拮抗卡那霉素加呋塞米诱导的小鼠SGNs损伤。
关键词
高血糖素样肽-1受体;艾塞那肽;小鼠;螺旋神经元
基金项目(Foundation):
国家自然科学基金青年科学基金项目(81900946);; 重庆市技术创新与应用发展专项重点项目(CSTC2021jscx-gksb-N0011)
作者
邬红霞;欧阳曦;范肖霞;戴光炆;赵旭旭;陈弢;康厚墉
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