胃蛋白酶在不同pH条件下对咽喉黏膜上皮的作用
The effects of pepsin on the mucosal epithelium of the laryngopharynx under different pH condition
郑彦焱;颜冬惠;陈十燕;龚宏勋;陈贤明
1:联勤保障部队第九〇〇医院耳鼻咽喉头颈外科
2:福建医科大学研究生部
3:福建医科大学福总临床医学院





[1] LECHIEN J R,AKST L M,HAMDAN A L,et al.Evaluation and management of laryngopharyngeal reflux disease:state of the art review[J].Otolaryngol Head Neck Surg,2019,160(5):762-782.
[2] LECHIEN J R,SAUSSEZ S,SCHINDLER A,et al.Clinical outcomes of laryngopharyngeal reflux treatment:a systematic review and meta-analysis[J].Laryngoscope,2019,129(5):1174-1187.
[3] LECHIEN J R,DAPRI G,DEQUANTER D,et al.Surgical treatment for laryngopharyngeal reflux disease:a systematic review[J].JAMA Otolaryngol Head Neck Surg,2019,145(7):655-666.
[4] SAVARINO E,DE BORTOLI N,ZENTILIN P,et al.Alginate controls heartburn in patients with erosive and none-rosive reflux disease[J].World J Gastroenterol,2012,18(32):4371-4378.
[5] LECHIEN J R,BOBIN F,MULS V,et al.Validity and reliability of the reflux symptom score[J].Laryngoscope,2020,130(3):E98-E107.
[6] LEE J S,JUNG A R,PARK J M,et al.Comparison of characteristics according to reflux type in patients with laryngopharyngeal reflux[J].Clin Exp Otorhinolaryngol,2018,11(2):141-145.
[7] LEE Y C,KWON O E,PARK J M,et al.Do laryngoscopic findings reflect the characteristics of reflux in patients with laryngopharyngeal reflux?[J].Clin Otolaryngol,2018,43(1):137-143.
[8] HURLEY B P,JUGO R H,SNOW R F,et al.Pepsin triggers neutrophil migration across acid damaged lung epithelium[J].Sci Rep,2019,9(1):13778-13778.
[9] GTARI W,BEY H,ASCHI A,et al.Impact of macromolecular crowding on structure and properties of pepsin and trypsin[J].Mater Sci Eng C Mater Biol Appl,2017,72:98-105.
[10] KOWALIK K,KRZESKI A.The role of pepsin in the laryngopharyngeal reflux[J].Otolaryngol Pol,2017,71(6):7-13.
[11] SAMUELS T L,ZIMMERMANN M T,ZEIGHAMI A,et al.RNA sequencing reveals cancer-associated changes in laryngeal cells exposed to non-acid pepsin[J].Laryngoscope,2021,131(1):121-129.
[12] BUCKLEY A,TURNER J R.Cell biology of tight junction barrier regulation and mucosal disease[J].Cold Spring Harb Perspect Biol,2018,10(1):a029314-a029314.
[13] MANDEL I,PAPERNA T,GLASS-MARMOR L,et al.Tight junction proteins expression and modulation in immune cells and multiple sclerosis[J].J Cell Mol Med,2012,16(4):765-775.
[14] OGURO M,KOIKE M,UENO T,et al.Dissociation and dispersion of claudin-3 from the tight junction could be one of the most sensitive indicators of reflux esophagitis in a rat model of the disease[J].J Gastroenterol,2011,46(5):629-638.
[15] SEREG-BAHAR M,JERIN A,JANSA R,et al.Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux—a prospective comparative study[J].Clin Otolaryngol,2015,40(3):234-239.
[16] ZIKOS T A,CLARKE J O.Non-acid reflux:when it matters and approach to management[J].Curr Gastroenterol Rep,2020,22(9):43.
[17] WOODLEY F W.Superimposed non-acid reflux event:an example of when it may be important to revisit the impe-dance analysis guidelines[J].J Neurogastroenterol Motil,2022,28(1):165-166.
[18] LI J,WANG J,WU M,et al.The role of nonacid reflux in laryngopharyngeal reflux diseases[J].Eur Arch Otorhinolaryngol,2020,277(10):2813-2819.
[19] KANEMITSU Y,KUROKAWA R,TAKEDA N,et al.Clinical impact of gastroesophageal reflux disease in patients with subacute/chronic cough[J].Allergol Int,2019,68(4):478-485.
[20] JOHNSTON N,DETTMAR P W,ONDREY F G,et al.Pepsin:biomarker,mediator,and therapeutic target for reflux and aspiration[J].Ann N Y Acad Sci,2018,1434(1):282-289.
[21] KIM J J,KIM N,PARK J H,et al.Comparison of tight junction protein-related gene mrna expression levels between male and female gastroesophageal reflux disease patients[J].Gut Liver,2018,12(4):411-419.
[22] TAN J J,WANG L,MO T T,et al.Pepsin promotes IL-8 signaling-induced epithelial-mesenchymal transition in laryngeal carcinoma[J].Cancer Cell Int,2019,19:64.
[23] JOHNSTON N,YAN J C,HOEKZEMA C R,et al.Pepsin promotes proliferation of laryngeal and pharyngeal epithelial cells[J].Laryngoscope,2012,122(6):1317-1325.
[24] LIESA M,SHIRIHAI O S.Mitochondrial dynamics in the regulation of nutrient utilization and energy expenditure[J].Cell Metab,2013,17(4):491-506.
[25] LECHIEN J R,MOUAWAD F,BARILLARI M R,et al.Treatment of laryngopharyngeal reflux disease:a syste-matic review[J].World J Clin Cases,2019,7(19):2995-3011.
[26] WALENTEK P,BEYER T,HAGENLOCHER C,et al.ATP4a is required for development and function of the xenopus mucociliary epidermis—a potential model to study proton pump inhibitor-associated pneumonia[J].Dev Biol,2015,408(2):292-304.