胃蛋白酶在不同pH条件下对咽喉黏膜上皮的作用

The effects of pepsin on the mucosal epithelium of the laryngopharynx under different pH condition

郑彦焱;颜冬惠;陈十燕;龚宏勋;陈贤明

1:联勤保障部队第九〇〇医院耳鼻咽喉头颈外科

2:福建医科大学研究生部

3:福建医科大学福总临床医学院

摘要
目的 探讨酸性及非酸性条件下胃蛋白酶对咽喉部黏膜上皮的损伤作用,为难治性咽喉反流性疾病治疗提供新思路。方法 30只健康豚鼠随机分为3组,每组10只:A组灌注胃蛋白酶+盐酸溶液(pH=2),B组灌注胃蛋白酶+蒸馏水(pH=7),C组灌注蒸馏水(pH=7)。灌注14 d后取环后区黏膜采用HE染色、免疫组化、透射电镜检测炎症细胞浸润程度、occludin和claudin-3蛋白表达、上皮细胞间隙增宽程度,电镜下分析细胞亚显微结构。结果 (1)A组黏膜下白细胞计数28.61±7.10个,B组29.89±7.04个,C组7.81±1.40个,A、B两组高于C组(P<0.05);(2)与C组相比较,A组occludin和claudin-3蛋白表达均下调(P<0.05),B组occludin和claudin-3蛋白表达差异无统计学意义(P>0.05);(3)电镜下A组黏膜上皮细胞间隙增宽,线粒体肿胀、嵴退化;B组黏膜上皮细胞膜完好,细胞间隙无增宽,但胞质内线粒体肿胀、嵴退化;C组黏膜上皮细胞间隙无增宽,线粒体形态标志正常。结论 与酸性条件下相比,非酸性条件下胃蛋白酶虽不引起细胞间隙增宽,但也可造成咽喉部黏膜上皮炎症性改变和线粒体损伤。
关键词
咽喉反流性疾病;胃蛋白酶;pH值;电镜
基金项目(Foundation):
福建省自然科学基金面上项目(2020J011143)
作者
郑彦焱;颜冬惠;陈十燕;龚宏勋;陈贤明
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