庆大霉素不同部位聚积引起的耳蜗外植体毛细胞梯度缺失模式

Gradient damage pattern of hair cell loss in cochlear explants induced by gentamicin

潘旭;宣伟军;丁大连

1:广西中医药大学

2:广西中医药大学第一临床医学院耳鼻咽喉头颈外科

3:纽约州立大学布法罗大学

摘要
目的 探讨庆大霉素诱导体外培养耳蜗外植体毛细胞的缺失模式。方法 用含0、0.1、0.5、1.0、1.5、3.0 mM庆大霉素的培养液体外培养耳蜗外植体(各组10个耳蜗)24 h后,应用FITC标记鬼笔环肽染色特异性显示耳蜗毛细胞的静纤毛,在荧光显微镜下观察耳蜗基底膜的毛细胞形态和数量。Rhodamine标记的0.5 mM庆大霉素的培养液培养耳蜗外植体12、24、36 h(各组10个耳蜗)后,在荧光显微镜下观察耳蜗不同位置庆大霉素的含量。结果 不同浓度庆大霉素培养耳蜗基底膜24 h后,0.1 mM庆大霉素对耳蜗毛细胞损伤甚微,0.5、1.0、1.5、3.0 mM庆大霉素均可造成耳蜗毛细胞损害,以底回毛细胞最严重,随着庆大霉素浓度升高,逐渐向顶回毛细胞发展。用0.5 mM庆大霉素培养后,庆大霉素最先积聚在底回外毛细胞,随培养时间增加逐渐向底回内毛细胞、顶回内外毛细胞蔓延,毛细胞损害程度也逐渐加重;且耳蜗外植体毛细胞的破坏部位和程度与荧光标记的庆大霉素在毛细胞中积聚的部位和程度相一致。结论 庆大霉素在不同部位内外毛细胞的积聚不同导致了耳蜗外植体毛细胞的梯度损伤模式,这可能与不同位置毛细胞的摄药能力差别有关。
关键词
庆大霉素;耳蜗;毛细胞;体外培养
基金项目(Foundation):
国家自然科学基金(81973913、81774374);; 研究生教育创新计划项目(YCBXJ2022017)
作者
潘旭;宣伟军;丁大连
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庆大霉素耳蜗毛细胞体外培养

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潘旭宣伟军丁大连