豚鼠耳蜗外侧壁周细胞的分布及形态学观察

Morphological study of pericytes in the lateral wall of cochlea in guinea pig

范术杭;塞娜;孙健斌;王琴;郭维维;韩维举

1:中国人民解放军医学院

2:中国人民解放军总医院第六医学中心耳鼻咽喉头颈外科医学部

3:听觉与平衡觉全国重点实验室

4:国家耳鼻咽喉疾病临床医学研究中心

摘要
目的 观察豚鼠耳蜗外侧壁周细胞的分布、形态结构并分析其功能特点,为耳蜗病理生理学研究提供参考。方法 显微镜下解剖分离豚鼠血管纹和螺旋韧带,利用HE染色、免疫荧光染色等对耳蜗外侧壁进行显微成像,观察周细胞的形态结构特征。结果 周细胞在血管纹及螺旋韧带上均有分布。血管纹边缘细胞形状规则、排列整齐,血管纹基底细胞呈不规则的扁平状。血管纹中间层毛细血管沿耳蜗螺旋走行,毛细血管间相互交织成网状,周细胞位于毛细血管网周围,多出现在毛细血管网交叉处;其形态不规则,细胞核多为椭圆形,细胞质形成的足突紧贴血管壁,与微血管长轴平行并逐渐分支变细,呈树枝样或点状包裹毛细血管。周细胞被血-迷路屏障内基底膜包裹,两者紧密相邻。结论 周细胞在血管纹和螺旋韧带中均有分布,广泛分布在毛细血管周围。周细胞的结构特征与其维持血-迷路屏障完整、调节耳蜗血流、维持血管稳定等重要生理功能相适应。
关键词
周细胞;血管纹;血-迷路屏障;形态学;耳蜗
基金项目(Foundation):
北京市自然科学基金项目(7244412、7242134);; 国家耳鼻咽喉疾病临床医学研究中心开放课题(202200007)
作者
范术杭;塞娜;孙健斌;王琴;郭维维;韩维举
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