饱和氢生理盐水对卡那霉素耳毒性保护作用的研究

Study on the protective effect of saturated hydrogen saline on kanamycin induced ototoxicity

陈远临;劳云燕;邢巍巍

1:锦州医科大学附属第一医院耳鼻咽喉头颈外科

摘要

目的研究饱和氢生理盐水(saturated hydrogen saline, SHN)对卡那霉素(kanamycin, KM)耳毒性的保护作用。方法将42只SPF级BALB/c雄性小鼠随机分为NS组(腹腔注射生理盐水10 ml/kg, 1次/天)、KM组(皮下注射卡那霉素750 mg/kg, 2次/天)和SHN组(腹腔注射饱和氢生理盐水10 ml/kg, 1次/天，同时皮下注射卡那霉素750 mg/kg, 2次/天),每组均连续用药14 d。各组给药前和给药结束后行听性脑干反应(auditory brainstem response, ABR)检测，检测耳蜗中总超氧化物歧化酶(total superoxide dismutase, T-SOD)活性和丙二醛(malondialdehyde, MDA)含量，扫描电镜(scanning electron microscopy, SEM)和琥珀酸脱氢酶(succinate dehydrogenase, SDH)染色观察耳蜗毛细胞。结果给药前，三组间各频率ABR反应阈差异无统计学意义(均为P>0.05)。给药后SHN组ABR反应阈和耳蜗中MDA含量均低于KM组(P<0.01),高于NS组(P<0.01); T-SOD活性高于KM组(P<0.01),低于NS组(P<0.01)。SEM和SDH染色结果显示，NS组毛细胞纤毛无粘连、紊乱和倒伏，毛细胞SDH区染色深；KM组毛细胞纤毛排列紊乱交错和倒伏明显，毛细胞SDH区几乎不着色；SHN组较KM组轻，仅有少部分毛细胞纤毛缺失、粘连、紊乱和倒伏。结论饱和氢生理盐水通过降低耳蜗中的氧化应缴损伤从而对卡那霉素耳毒性具有保护作用。

关键词

饱和氢生理盐水;卡那霉素;抗氧化;氧化应激

基金项目(Foundation):

作者

陈远临;劳云燕;邢巍巍

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饱和氢生理盐水卡那霉素抗氧化氧化应激

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陈远临劳云燕邢巍巍