右美托咪定对小鼠大脑皮层听性稳态反应的影响

Effects of Dexmedetomidine on the Auditory Steady State Response in Mouse

李金红;李子杰;王帅;秦岭;

1:中国医科大学生命科学学院听力研究室生理学教研室

摘要
目的 检测右美托咪定(dexmedetomidine, DEX)对小鼠听性稳态反应(auditory steady-state response, ASSR)的影响,以探讨DEX对大脑感觉中枢神经电活动的调节作用。方法 选用8只6~8周龄C57BL/6小鼠,全身麻醉下,在小鼠听皮层(auditory cortex, AC)区进行慢性电极植入手术,恢复1周后,使用在体电生理记录方法,分别记录每只小鼠在自由清醒状态下和腹腔注DEX(80μg/kg)后,对40 Hz click-trains声音刺激的ASSR变化;用平均能量(mean trial power, MTP)和锁相因子(phase-locking factor, PLF)对ASSR进行定量评估。结果 腹腔注射DEX后可以显著降低40Hz ASSR的MTP和PLF(t7=2.858,P<0.05;t7=2.507,P<0.05)。结论 DEX诱导的镇静效应可能与大脑皮层神经元之间同步活动减少相关,而ASSR可用于监测DEX镇静效果的潜在生物标志物。
关键词
右美托咪定;α2-受体激动剂;听性稳态反应;听觉皮层
基金项目(Foundation):
国家自然基金面上项目(31671080)
作者
李金红;李子杰;王帅;秦岭;
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