单核细胞趋化蛋白-1及脂蛋白(a)与梅尼埃病关系的研究

The Relationship between Monocyte Chemotactic Protein-1 and Lipoprotein (a) and Meniere's Disease

王娜;李旻;王静;李伟兰;刘汝利;张再兴;周尚清;郭霞;

1:华北理工大学附属医院耳鼻咽喉头颈外科

2:华北理工大学附属医院骨外科

3:河北省定州市人民医院康复科

4:华北理工大学附属医院影像科

摘要
目的探讨单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)及脂蛋白(a)[lipoprotein (a),LP(a)]与梅尼埃病的关系。方法选取30只健康SD大鼠,分成正常组、对照组及实验组,每组10只,实验组腹腔注射醋酸去氨加压素(4μg·kg(-1)·d(-1)·d(-1)连续注射7 d后改为6μg·kg(-1)连续注射7 d后改为6μg·kg(-1)·d(-1)·d(-1)连续注射3 d)行膜迷路积水造模,对照组腹腔注射等量生理盐水,正常组不作处理,停药7 d后对三组动物行ABR检测,取耳蜗中阶横截面积(SM)/(中阶+前庭阶横截面积)(SM+SV)比值评价膜迷路积水情况。造模成功后通过免疫组化染色检测耳蜗组织中MCP-1的表达,通过酶联免疫吸附实验(ELISA)法检测血清中LP(a)的水平,对LP(a)水平与内耳积水程度及大鼠ABR各指标进行相关性分析。结果实验组波Ⅱ阈值升高、波Ⅱ、Ⅴ潜伏期及Ⅲ-Ⅴ波间期较正常组和对照组均延长,差异有统计学意义(P<0.05)。实验组、对照组、正常组SM/(SM+SV)比值分别为0.45±0.04、0.34±0.02、0.35±0.01,前者显著大于后两者(P<0.05)。实验组MCP-1在耳蜗螺旋器、血管纹螺旋韧带及神经节细胞组织表达明显,前庭膜上也有表达,其它两组无表达。实验组、对照组、正常组LP(a)水平分别为4.07±0.20、1.36±0.12、1.38±0.14 mg/L,前者显著高于后两者(P<0.001),LP(a)表达水平与膜迷路积水程度及ABR各指标间均呈正相关(P<0.001)。结论膜迷路积水模型大鼠耳蜗组织中MCP-1表达明显,血清LP(a)水平明显升高,且LP(a)水平与膜迷路积水程度及大鼠听力下降呈正相关,提示MCP-1和LP(a)可能与梅尼埃病的发生有关。
关键词
梅尼埃病;单核细胞趋化蛋白-1;脂蛋白(a)
基金项目(Foundation):
河北省科技厅重点研发计划项目(172777175);; 河北省医学科学研究课题计划项目(20191111);; 华北理工大学科学研究基金项目(Z201729)
作者
王娜;李旻;王静;李伟兰;刘汝利;张再兴;周尚清;郭霞;
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