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Tnn基因对小鼠听功能影响的研究

Effect of Tnn on Hearing Function in Mouse

陆菲;吴玉丽;范贝;刘珍珍;梁鹏飞;李薇;查定军;

1:空军军医大学第一附属医院耳鼻咽喉头颈外科

2:陕西省第四人民医院

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摘要

目的探讨Tnn基因对小鼠听觉功能的影响。方法 (1)取2月龄、3月龄的C57BL/6野生型(wild-type, WT)小鼠各3只进行耳蜗冰冻切片及免疫荧光染色,观察Tnn基因在小鼠耳蜗的表达;(2)各取8只野生型WT小鼠及Tnn基因敲除小鼠(Tnn(+/-)小鼠)在1月龄、2月龄、3月龄进行短声(click)及短纯音(tome burst, tb),听性脑干反应(ABR)测试并比较结果。结果 (1)免疫荧光显示Tnn基因在2月龄、3月龄WT小鼠耳蜗中均有表达,且主要表达于螺旋神经节神经元,与Ⅲ型β-tubulin共定位;(2)Tnn(+/-)小鼠)在1月龄、2月龄、3月龄进行短声(click)及短纯音(tome burst, tb),听性脑干反应(ABR)测试并比较结果。结果 (1)免疫荧光显示Tnn基因在2月龄、3月龄WT小鼠耳蜗中均有表达,且主要表达于螺旋神经节神经元,与Ⅲ型β-tubulin共定位;(2)Tnn(+/-)小鼠在3月龄时,click-ABR阈值较WT型升高小鼠(分别为41.88±9.64和23.13±2.59 dB SPL,P<0.01),tb-ABR阈值4、8、16、24、32 kHz(分别为45.00±17.73、44.38±18.60、45.63±18.60、50.00±20.70、54.38±19.90 dB SPL)较WT小鼠(分别为21.25±2.31、21.87±2.59、21.25±2.31、22.50±2.67、24.38±1.77 dB SPL)均升高(P<0.05或P<0.01)。结论 Tnn基因对维持小鼠听功能有重要作用,该蛋白表达异常可使小鼠听功能受损,推测其可能影响螺旋神经节神经元的正常发育,影响神经元细胞的迁移或神经突向外生长,从而引起听觉功能的异常。

关键词

腱糖蛋白-W;Tnn基因;耳蜗;基因敲除

基金项目(Foundation):

国家自然科学基金项目（81870732 81800918）;; 陕西省科技重点项目（2017ZDXM-SF-061,2018PT-01）;; 西京医院助推项目（XJZT18MDT07）

作者

陆菲;吴玉丽;范贝;刘珍珍;梁鹏飞;李薇;查定军;

参考文献

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