腱糖蛋白-W在不同发育阶段C57BL/6小鼠耳蜗中的表达

Tenascin-W Expression in the Cochleae at Different Stages of Development in C57BL/6 Mice

陆菲;雷文静;范贝;梁鹏飞;李薇;陈俊;查定军;

1:空军军医大学第一附属医院耳鼻咽喉头颈外科

摘要
目的研究Tnn基因编码的腱糖蛋白-W(tenascin-W,TN-W)在出生后不同发育阶段小鼠耳蜗内的表达,初步探讨tenascin-W在听觉系统中的功能。方法以出生后(postnatal day,P)1、3、14、28天(P1、P3、P14、P28)各6只及出生后7天(P7)9只C57BL/6小鼠作为研究对象,通过免疫荧光标记观察tenascin-W在耳蜗中的表达及定位,运用实时荧光定量PCR(real-time quantitative PCR, RT-qPCR)检测各发育阶段小鼠耳蜗中Tnn mRNA相对表达水平,Western blot法检测tenascin-W在小鼠耳蜗中的表达。结果免疫荧光标记显示tenascin-W在P1、P3、P7、P14、P28小鼠耳蜗中均有表达。在P1、P3、P7小鼠耳蜗螺旋神经节、Corti器中均有表达,在P14、P28小鼠仅在螺旋神经节高表达,在Corti器中不表达,在各发育阶段小鼠耳蜗血管纹均有表达;共染显示其与Ⅲ型β-tubulin共定位在螺旋神经节神经元胞体的胞浆中。RT-qPCR显示Tnn mRNA在P7小鼠耳蜗相对表达量最高,Western blot法检测到tenascin-W在P7小鼠耳蜗中有表达。结论 Tenascin-W在小鼠耳蜗各发育阶段均有表达,在P7表达量最高,且主要定位于螺旋神经节神经元,提示该蛋白可能影响耳蜗螺旋神经元发育及功能,对小鼠正常听觉的维持发挥重要作用。
关键词
腱糖蛋白-W;Tnn基因;耳蜗;小鼠;近交C57BL/6
基金项目(Foundation):
国家自然科学基金项目(81870732);; 陕西省科技重点项目(2017ZDXM-SF-061,2018PT-01);; 西京医院助推项目(XJZT14X07)
作者
陆菲;雷文静;范贝;梁鹏飞;李薇;陈俊;查定军;
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