大鼠耳蜗传出神经系统发育中降钙素基因相关肽和乙酰胆碱酯酶的时空分布

Growth and Development Process of Calcitonin Gene-related Peptide and Acetylcholinesterase in Rat Cochlear Efferent Nervous System

丁大连;张建辉;杨琨;蒋海燕;亓卫东;时海波;刘芳;李鹏;Richard Salvi;

1:Center for Hearing and Deafness

2:State University of New Yorkat Buffalo

3:成都市第三人民医院耳鼻咽喉头颈外科

4:上海市第六人民医院耳鼻咽喉头颈外科

摘要
目的探讨耳蜗发育过程中传出神经的发育分布。方法应用Tublin免疫组织化学方法标记出生后3~15天以及出生后4个月大鼠耳蜗基底膜上所有传入和传出神经纤维及其末梢的分布;应用酶组织化学或免疫组织化学方法观察出生后不同时间耳蜗基底膜上降钙素基因相关肽(calcitonin gene-related peptide,CGRP)和乙酰胆碱脂酶(AchE)的表达和分布。结果耳蜗传入神经纤维及末梢在大鼠出生后当天已经与内、外毛细胞建立起突触联系;从出生后第五天开始CGRP出现在内毛细胞周围及底部,但AchE标记的传出神经尚未进入Corti器,出生后第七天AchE才开始与CGRP同时出现在内侧螺旋束和隧道螺旋束的耳蜗传出神经纤维及末梢。结论 CGRP和乙酰胆碱出现在Corti器的时间均明显早于耳蜗传出神经末梢在耳蜗靶细胞的就位;在大鼠耳蜗发育早期出现在内毛细胞区域的CGRP和乙酰胆碱或许是由内毛细胞及其周围支持细胞分泌的一种诱导耳蜗传出神经纤维朝着耳蜗螺旋器趋向性生长的引导信号。
关键词
耳蜗发育;耳蜗传出神经;降钙素基因相关肽;乙酰胆碱酯酶
基金项目(Foundation):
作者
丁大连;张建辉;杨琨;蒋海燕;亓卫东;时海波;刘芳;李鹏;Richard Salvi;
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