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CO_2激光犬声带全切除后不同时间细胞外基质表达

Expression of Extracellular Matrix at Different Periods after CO_2 Laser Complete Removal of Vocal Cords in Canine

汪斌如;张莹莹;杨丽萍;刘莉;陈始明;梁耕田;

1:武汉大学附属同仁医院(武汉市第三医院)耳鼻咽喉头颈外科

2:武汉大学人民医院耳鼻咽喉头颈外科

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摘要

目的观察犬声带损伤后不同自愈时间段细胞外基质(extracellular matrix,ECM)相关影响因子的动态变化规律,探讨声带损伤后的可能修复机制。方法 5只实验犬分为实验组(4只,8侧声带)和对照组(1只,2侧声带)。实验组在支撑喉镜下暴露双侧声带,CO_2激光6焦耳/秒行双侧声带全切除深达肌层,对照组在支撑喉镜下观察声带形态后不作任何处理。实验犬于双侧声带CO_2激光损伤前及损伤后6 h、3 w、8 w和12 w分别进行喉镜检查声带创面愈合情况,然后,每个时间点取1只犬的声带标本行HE染色、免疫荧光染色检查,同时检测ECM中赖氨酰氧化酶(LOX)、基质金属蛋白酶-1(MMP-1)和缺氧诱导因子-1α(HIF-1α)的累积光密度值(IOD值)。结果 (1)喉镜检查示,术后3 w实验组犬声带创面仍有充血水肿,术后8 w声带创面光滑凹陷,术后12 w声带局部挛缩。(2)HE染色显示,术后6 h实验组声带创面大量红细胞和炎性细胞浸润;术后3 w创面成纤维细胞增生,新生血管形成;术后8 w创面纤维组织增生紊乱;术后12 w声带创面胶原纤维大量增生,排列紊乱,间隙增大。(3)免疫荧光染色显示,术后6 h和3 w,实验组声带创面炎性细胞、血管内皮细胞和腺体内LOX、MMP-1和HIF-1α表达较强烈;术后8 w和12 w,创面阳性细胞逐渐减少,随着创面愈合其表达趋于稳定。(4)各时间点LOX和MMP-1的IOD值比较差异均有统计学意义(P<0.05);除术后12 w与术前比较外,其余各时间点间HIF-1α的IOD值差异均有统计学意义(P<0.05)。结论 CO_2激光犬声带全切除后12 w内,ECM中LOX、MMP-1和HIF-1α差异化表达可能与声带的创伤修复及瘢痕形成有关。

关键词

声带;声带损伤;细胞外基质

基金项目(Foundation):

湖北省自然科学基金项目(2018CFB455);; 湖北省卫生健康委员会科研项目(WJ2019M007)

作者

汪斌如;张莹莹;杨丽萍;刘莉;陈始明;梁耕田;

参考文献

1 Rousseau B,Hirano S,Scheidt TD,et al.Characterization of vocal fold scarring in a canine model[J].Laryngoscope,2003,113:620.

2 Branski RC,Rosen CA,Verdolini K,et al.Acute vocal fold wound healing in a rabbit model[J].Ann Otol Rhinol Laryngol,2005,114:19.

3 Friedrich G,Dikkers FG,Arens C,et al.Vocal fold scars:current concepts and future directions.Consensus report of the phonosurgery committee of the european laryngological society[J].Ear Arch Otorhinolaryngol,2013,270:2491.

4 Graupp M,Bachna RS,Gerstenberger C,et al.The unsolved chapter of vocal fold scars and how tissue engineering could help us solve the problem[J].Eur Arch Otorhinolaryngol,2016,273:2279.

5 梁耕田,段冰玉,张莹莹,等.犬声带瘢痕模型的建立及其细胞外基质中赖氨酰氧化酶、热休克蛋白70和透明质酸表达的研究[J].临床耳鼻咽喉头颈外科杂志,2017,31:116.

6 Xie J,Jiang J,Zhang Y,et al.Up-regulation expressions of lysyl oxidase family in anterior cruciate ligament and medial collateral ligament fibroblasts induced by transforming growth factor-beta 1[J].Int Orthop,2012,36:207.

7 Moses MA,Marikovsky M,Harper JW,et al.Temporal study of the activity of matrix metalloproteinases and their endogenous inhibitors during wound healing[J].J Cell Biochem,2015,60:379.

8 Mace KA,Yu DH,Paydar KZ,et al.Sustained expression of Hif-1α in the diabetic environment promotes angiogenesis and cutaneous wound repair[J].Wound Repair Regen,2010,15:636.

9 Sivakumar P,Gupta S,Sarkar S,et al.Upregulation of lysyl oxidase and MMPs during cardiac remodeling in human dilated cardiomyopathy[J].Mol Cell Biochem,2008,307:159.

10 Wang WM,Ma XJ,Huang SB,et al.A comparative study of effect of autograft versus allograft anterior cruciate ligament reconstruction on expressions of LOXs and MMPs[J].Bioscience Rep,2017,37:1.

11 Xie J,Wang C,Yin L,et al.Interleukin-1 beta influences on lysyl oxidases and matrix metalloproteinases profile of injured anterior cruciate ligament and medial collateral ligament fibroblasts[J].Int Orthop,2013,37:495.

12 徐文,韩德民,胡蓉,等.声带损伤后自身修复特点研究[J].首都医科大学学报,2011,32:729.

13 王春莉,梅虎,谢静,等.共培养下前交叉韧带成纤维细胞中LOXs与MMPs的基因表达情况[J].生物医学工程学杂志,2013,30:105.

14 Hansen JK,Thibeault SL.Current understanding and review of the literature:vocal fold scarring[J].J Voice,2006,20:110.

15 Hirano S,Bless DM,Rousseau B,et al.Fibronectin and adhesion molecules on canine scarred vocal folds[J].Laryngoscope,2003,113:966.

16 Rouseseau B,Hiranos,Chan RW,et al.Characteri zation of chronic vocal fold scarring in a rabbit model[J].J Voice,2004,18:116.

17 王家佳,魏春生.声带瘢痕的形成及治疗进展[J].听力学及言语疾病杂志,2011,19:283.

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